Culture method and application of primary amniotic stem cells

A culture method and stem cell technology, which is applied to animal cells, embryonic cells, vertebrate cells, etc., can solve the problems of low cell purity, difficulty in cell attachment, and complicated removal, so as to accelerate cell attachment, shorten culture time, and improve cell The effect of improving the purity
CN114686426APending Publication Date: 2022-07-01SHENZHEN WINGOR BIO TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHENZHEN WINGOR BIO TECH
Publication Date
2022-07-01

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Abstract

The invention belongs to the technical field of stem cell culture, and particularly relates to a culture method and application of primary amniotic stem cells. The culture method of the primary amniotic stem cells mainly comprises the following steps: stripping a placenta under a sterile condition, washing with normal saline, washing with a mixed solution containing a bactericide, treating with digestive enzyme, culturing with a culture medium and the like. The mixed solution containing the bactericide is prepared from the following components in percentage by weight: 1 to 2 percent of herba taraxaci extract, 0.5 to 0.8 percent of rosemary hydrolat, 0.3 to 0.8 percent of vitamin C and 96.4 to 98.2 percent of purified water; the culture medium is composed of an LB liquid culture medium, tween-20, type IV collagen and BI recombinant vitreous adhesion protein. By adopting the culture method provided by the invention, cell adherence can be accelerated, tissues in the cells can be cleaned up, and the cell purity is improved on the premise of shortening the culture time of the amniotic stem cells.
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Description

technical field

[0001] The invention belongs to the technical field of stem cell culture, and in particular relates to a culture method of primary amniotic stem cells and application thereof. Background technique

[0002] Human amniotic membrane-derived stem cells include human amniotic epithelial cells (humanamniotic epithelial cells, hAEC) and human amniotic mesenchymal stem cells (human amnioticmes-enchymalstromal cells, hAMSC). Among them, human amniotic epithelial cells have the characteristics of expressing a variety of embryonic stem cell markers and have a more comprehensive multi-directional differentiation potential. hMSCs have stronger proliferation ability and stem cell characteristics than bone marrow mesenchymal stem cells. Therefore, due to its totipotency, amniotic stem cells are often used in various cytology and molecular biology research processes.

[0003] However, the existing amniotic membrane stem cell culture method is difficult to adhere to due to t...

Claims

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