Construction and application of transgenic mouse model for exosome tracing

A technology of transgenic mice and exosomes, applied in the construction of transgenic mouse models, can solve the problems of silencing, reading frame changes, loss of function of target genes, etc.
CN114686518APending Publication Date: 2022-07-01NANJING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING UNIV
Publication Date
2022-07-01

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Abstract

The invention discloses a construction method and application of a transgenic mouse model for exosome tracing. The specific implementation process mainly comprises the following steps: constructing plasmids for expressing stop-EGFP-tracrRNA and expressing crRNA or sgRNA; construction of donor cells and verification of exosomes; constructing, breeding and identifying transgenic mice; the five parts are verified by the tracing exosome transgenic mouse model, and finally the transgenic mouse model for tracing the exosome is successfully obtained; the successful construction of the model lays a foundation for the research of the exosome as a gene drug carrier, and scientists can more easily and clearly observe the distribution of the exosome in vivo under pathological or physiological conditions.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular, to a construction method and application of a transgenic mouse model for exosome tracking. Background technique

[0002] The CRISPR-Cas system is a natural immune system of prokaryotes. After some bacteria or archaea are invaded by viruses, they can store a small segment of the viral gene in a storage space called CRISPR in their own DNA; When a virus invades, bacteria can identify the virus according to the stored and written segments, and cut off the DNA of the virus to make it invalid; the mechanism of CRISPR-Cas9 is to drive immunity, and its mechanism of action can be divided into three stages to understand 1. Acquisition: Immunity is generated by ingesting exogenous DNA sequences and integrating them into new CRISPR spacers; 2. Expression: CRISPR sequences are transcribed under the control of the leader region to generate pre-crRNA (precursor of crRNA), which is compatible with p...

Claims

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