Multi-dimensional liquid chromatography device and method for purifying ginkgetin
A technology of multi-dimensional liquid chromatography and ginkgo flavonoids, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of insufficient competitiveness in the international market, and achieve the effects of improving competitiveness, high efficiency, and high yield
Pending Publication Date: 2022-07-05
SOOCHOW HIGH TECH CHROMATOGRAPHY
View PDF0 Cites 1 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
The total demand for Ginkgo biloba extract in the international market is about 500-700 tons. Although the domestic production of Ginkgo biloba is increasing every year, the competitiveness in the international market is insufficient.
[0005] At present, the industrial purification process mostly adopts macroporous resin column chromatography to purify Ginkgo biloba extract, and its purity is about 24% (Han Xuezhe, Wang Dongshuang, An Xiaodong, etc. Research on Ginkgo Flavonoids Extraction and Refining Technology[J].Chemical Engineer, 2011, 25 (4): 3.), fine purification is also based on monoflavonoids, and there is a lack of a device and method that can finely purify ginkgo single flavonoids and ginkgo double flavonoids at the same time
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0057] The following specific examples are further elaborated;
[0058]Numerous specific details are set forth in the following description to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways than those described herein, and thus the present invention is not limited by the specific embodiments disclosed below.
[0059] like figure 1 and figure 2 As shown, a multidimensional liquid chromatography device for purifying ginkgo flavone includes a first pump system 1, a second pump system 2, a first chromatographic column switching valve 11, a chromatographic column array, a second chromatographic column switching valve 12, a first The trapping column switching valve 13 , the trapping column array, the second trapping column switching valve 14 , the fraction collector 6 , the waste liquid pool 7 , the detector 8 , the mixer 9 and the two-position ten-way valve 10 .
[0060] The chromatographic column array i...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention belongs to the technical field of chemical purification, and relates to a multidimensional liquid chromatography device for purifying ginkgetin, which comprises a first pump system, a second pump system, a chromatographic column array, a trapping column array, a fraction collector, a waste liquid pool, a detector, a mixer and a two-position ten-way valve, the two-position ten-way valve is provided with a position A for sequentially communicating the first pump system, the chromatographic column array, the detector, the mixer, the trapping column array, the fraction collector and the waste liquid pool, and a position B for sequentially communicating the first pump system, the trapping column array, the chromatographic column array, the detector, the mixer, the fraction collector and the waste liquid pool. The invention also provides a method for purifying ginkgo flavone, which comprises the following steps: loading a ginkgo leaf extracting solution to the first chromatographic column, and enriching a target fraction into the trapping column array for first-dimensional chromatographic separation; the target fractions in the elution trapping column array are sequentially loaded to a second chromatographic column for two-dimensional purification, second-dimensional target fraction eluent is collected and dried, and a product is obtained.
Description
technical field [0001] The invention belongs to the technical field of chemical purification, and relates to a liquid chromatography device and method, in particular to a multidimensional liquid chromatography device and method for purifying ginkgo flavone. Background technique [0002] Ginkgo biloba is the dried leaves of Ginkgo biloba, which mainly contains flavonoids and terpenoids. Ginkgo biloba flavonoids can dilate blood vessels, increase coronary and cerebral blood flow, reduce blood viscosity, and improve cerebral circulation. It is an effective drug for the treatment of cardiovascular diseases. [0003] Ginkgo flavonoids can be divided into 3 categories according to their structure: monoflavonoids are mainly quercetin, kaempferol, isorhamnetin, etc.; diflavonoids are mainly ginkgo biflavonoids, isoginkgo biflavonoids, golden pine biflavonoids, etc.; catechins Vegetarians mainly include catechin, epicatechin, gallic acid, epigallic acid and so on. [0004] Ginkgo p...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N2030/065
Inventor 王宝雷陈育拼彭凯翟钰霖胡荣敏
Owner SOOCHOW HIGH TECH CHROMATOGRAPHY