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Application of attenuated salmonella in preparation of medicine for treating lung cancer pleural effusion

A Salmonella and pleural effusion technology, applied in the application field of attenuated Salmonella in the preparation of pleural effusion drugs for the treatment of lung cancer, to achieve the effect of increasing survival time, improving pleural effusion, and improving quality of life

Pending Publication Date: 2022-07-08
JIANGSU TARGET BIOMEDICINE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Attenuated Salmonella has the characteristics of good safety and single administration, but the current clinical treatment for pleural effusion lacks the characteristics of high safety, single administration, and effective improvement of patients' quality of life and overall survival time. drug

Method used

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  • Application of attenuated salmonella in preparation of medicine for treating lung cancer pleural effusion
  • Application of attenuated salmonella in preparation of medicine for treating lung cancer pleural effusion
  • Application of attenuated salmonella in preparation of medicine for treating lung cancer pleural effusion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Construction of mouse lung cancer pleural effusion model

[0047] Experimental cells:

[0048] LLC cells were donated by the School of Life Sciences, Nanjing University.

[0049] Experimental animals:

[0050] Wild-type C57 mice (C57BL / 6J wild-type mice): SPF grade, 6-8 weeks old, mass 16-20 g, purchased from Changzhou Cavens Laboratory Animal Co., Ltd.

[0051] Main experimental equipment:

[0052] Bacterial culture shaker was purchased from Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.; pipette was purchased from Glison Company; desktop common centrifuge, desktop low-temperature refrigerated centrifuge and biological spectrophotometer were purchased from Eppendorf Company; PH instrument was purchased from Sartorius Company; Nucleic acid electrophoresis and protein vertical electrophoresis system were purchased from Bio-Rad Company of the United States; PCR instrument was purchased from Takara Company of Japan; autoclave was purchased from Zealway ...

Embodiment 2

[0060] In vitro growth curve determination of attenuated Salmonella VNP20009

[0061] The VNP20009 stored at -80°C was inoculated into LB liquid medium, recovered and cultured overnight in a bacterial incubator at 37°C, and then transferred to fresh LB at 1:100 the next day and cultured to an OD of 0.6-0.8. Take the same amount of VNP20009 strain. Transfer to an equal amount of fresh LB and place it in a 37°C bacterial incubator for 7 hours, sample once every 0.5 hours from the time the bacteria were transferred to LB (ie 0h), and use a biospectrophotometer to measure the OD600 nm of the bacteria value, plot the bacterial growth curve ( figure 2 ).

[0062] Attenuated Salmonella VNP20009 in the treatment of pleural effusion in mice with lung cancer and pleural effusion

[0063] Take lung cancer pleural effusion model mice, use isoflurane anesthesia machine to lightly anesthetize, use forceps to pick up the skin of the 5-6 intercostal space on the left side of the sternum of...

Embodiment 3

[0069] Construction of mouse lung cancer pleural effusion model

[0070] (1) In vitro cell culture

[0071] The LLC cells were taken out from a liquid nitrogen tank or -80°C refrigerator, thawed at 37°C for 1 min to recover the cells, centrifuged at 1000 rpm / min for 3 min, and cultured in 90% DMEN high-glucose medium and 10% fetal bovine serum in a 37°C 5% carbon dioxide incubator. After the cells entered the exponential growth phase and covered the bottom of the culture flask, the iron-walled cells were digested with trypsin and washed out with PBS. The total number of cells using the hemocytometer technique was used, and the proportion of viable cells was calculated using the trypan blue detection kit. When the proportion of viable cells reached more than 90%, the LLC cell suspension was diluted to 1x10 with normal saline. 6 spare, figure 1 .

[0072] (2) Construction of mouse lung cancer pleural effusion model

[0073] Take 30 6-8 week old C57BL / 6J wild-type mice, half ...

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Abstract

The invention discloses application of attenuated salmonella in preparation of a medicine for treating lung cancer pleural effusion. After administration, pleural effusion of a lung cancer pleural effusion mouse model is remarkably improved, the lifetime of the mouse is prolonged, and a new method is provided for clinical treatment of advanced lung cancer patients with pleural effusion symptoms.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of an attenuated Salmonella in the preparation of a medicine for treating lung cancer pleural effusion. Background technique [0002] Pleural effusion is one of the clinical events of advanced lung cancer, which seriously affects the quality of life of patients and clinical drug treatment. With the continuous deepening of my country's national economic reform and the acceleration of urbanization and industrialization, the incidence and mortality of lung cancer have increased, and now it has become the malignant tumor with the highest morbidity and mortality. According to statistics, in 2020, the total number of lung cancer cases in my country will reach 820,000, and the total number of deaths will reach 470,000. Advanced lung cancer has caused a huge physical and economic burden to patients and their families. In particular, the occurrence of pleural effusion often s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K9/00A61K9/19A61P35/00A61P7/10
CPCA61K35/74A61K9/0019A61K9/19A61P35/00A61P7/10Y02A50/30
Inventor 华子春郁文亮
Owner JIANGSU TARGET BIOMEDICINE RES INST
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