Method for promoting artificial rapid cultivation of biological crust by using functional microbial agent

A technology of biological crusting and artificial cultivation, applied in the field of desertification control and ecological environment restoration, can solve the problems of not improving the growth efficiency of biological crusts, speed up the biological crusts, etc. The effect of improved wind erosion resistance

Pending Publication Date: 2022-07-15
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth efficiency of biocrusts has not been improved in these technologies, and in order to promote the widespread application of artificial algal crust biotechnology in ecological restoration and desertification control projects, the formation of biocrusts must be accelerated

Method used

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  • Method for promoting artificial rapid cultivation of biological crust by using functional microbial agent
  • Method for promoting artificial rapid cultivation of biological crust by using functional microbial agent
  • Method for promoting artificial rapid cultivation of biological crust by using functional microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This embodiment provides a method for preparing a biological crust culture substrate and a biological crust seed powder, and the specific steps are as follows.

[0043] (1) Biocrust culture substrate

[0044] The present invention implements the collection of aeolian sand with a depth of 0-5cm on the surface of the mobile dune, and removes debris such as stones and vegetation roots through a 2mm sieve. A part of the samples were taken for the determination of pH value, organic carbon, available nitrogen, available phosphorus and available potassium content of bare sand. The remainder is used as a culture medium. In this example, aeolian sandy soil on the surface of moving sand dunes was selected as the culture substrate for biological crusts.

[0045] (2) Biocrust provenance

[0046] In this example, well-developed crusts were collected from different sand-fixing shrub woodlands and dunes at different locations, and the crusts with a thickness of 0-2 cm on the surfac...

Embodiment 2

[0051] The effect of embodiment 2 microbial inoculum on the growth of biological crusts

[0052] In this example, the biological crust provenance prepared in Example 1 was used, and the biological crust provenance was diluted with sterile distilled water (the inoculation amount was 100 g biological crust provenance / m 2 ), evenly inoculated on the surface of the aeolian sand (the aeolian sand was prepared by the method of Example 1) in the culture plate. In order to ensure uniform inoculation, the water far exceeding the water content of bare sand was first poured into the culture plate, and then the well-stirred medium containing the provenance was quickly sprayed into the water in the upper layer of the soil sample ( figure 1 ), after the excess water flows out from the small holes at the bottom of the culture plate, the biological crusts are evenly distributed on the surface of the aeolian sand ( figure 1 ).

[0053] The functional bacterial agents (carbon fixation, nitrog...

Embodiment 3

[0061] This embodiment provides a method for promoting the artificial rapid cultivation of biological crusts by a functional microbial inoculant.

[0062] This example adopts the biological crust culture substrate and biological crust seed source prepared in Example 1.

[0063] (1) Propagation using BG11 medium

[0064] Add aeolian sandy soil to the culture plate to make the aeolian sandy soil evenly cover the bottom of the plate, then add the biological crust seed powder prepared in Example 1 (add 200g of biological crust seed powder per liter of medium), stir evenly and pour it into the In a culture plate, cultured at room temperature and under natural light. After 21 days, the cultured biocrusts and the sand grains they fixed were collected together, air-dried, ground, and passed through a 1mm sieve to obtain the biocrust seed source. Store in a sterilized ziplock bag and store at -20°C.

[0065] (2) Artificial cultivation of biological crusts

[0066] The propagated bio...

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Abstract

The invention relates to the field of desertification control and ecological environment restoration, and provides a method for promoting artificial rapid cultivation of biological crust by using a functional microbial agent. The method provided by the invention comprises the following steps: uniformly inoculating a propagated biological crust seed source on a flowing sand surface, and adding a functional microbial inoculum to the flowing sand surface inoculated with the biological crust seed source according to a mass ratio of the inoculation amount of the biological crust seed source to the addition amount of the functional microbial inoculum of (1-1.2): (0.8-1). According to the method provided by the invention, the biological crust with high coverage, high biomass and high compressive strength can be formed within 30 days, and the formed biological crust can remarkably improve the stability of the sand surface, increase the soil fertility and accelerate the soil forming process of the desertification land, so that the method is an effective method for ecological restoration of the sand land.

Description

technical field [0001] The invention relates to the field of desertification control and ecological environment restoration, in particular to a method for promoting artificial rapid cultivation of biological crusts by a functional microbial inoculant. Background technique [0002] Climate change and human activities have led to aggravation of desertification, which has become one of the serious social, economic and environmental problems in many parts of the world. Depending on the drivers and geographic context, desertification may lead to increased bare soil, loss of soil productivity (eg, nutrient loss, reduction of soil fines and water-carrying capacity), increased soil salinity and toxicity, or changes in vegetation composition. Given the serious consequences of desertification, it is imperative to control and restore degraded lands in desert ecosystems so that the ecosystems can continue to function and provide ecological services that sustain human existence. Therefo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40C12N1/20C12N1/16C12N1/14C12N1/12C12R1/125C12R1/07C12R1/38C12R1/645C12R1/89C09K105/00
CPCC09K17/40C12N1/20C12N1/16C12N1/14C12N1/12C09K2105/00
Inventor 冯薇张宇清秦树高毛赫楠赖宗锐佘维维刘靓
Owner BEIJING FORESTRY UNIVERSITY
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