Application of ferrous glycinate in treatment of rheumatoid arthritis
A ferrous glycinate, rheumatoid technology, applied in the medical field, to improve swelling and destruction, avoid invasion of cartilage, and reduce the level of
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0052] Example 1 In vitro protective effect of ferrous glycinate on Raw264.7 cell inflammatory injury model induced by LPS and IFN-γ co-stimulation
[0053] 1. Raw264.7 cell culture
[0054] After the Raw264.7 cells were recovered, they were cultured in a petri dish with a diameter of 10 mm, and the medium was DMEM medium containing 10% fetal bovine serum (DMEM is a medium containing various amino acids and glucose, which was purchased from Thermo Fisher Scientific), the petri dish was placed at 37°C and 5% CO 2 Cultured in a constant temperature incubator, the medium was replaced every two days, and the cells in the culture dish could be subcultured when the density reached about 80%. Follow-up experiments were carried out after culturing for 3 to 5 generations according to conventional culture techniques in the art.
[0055] 2. Experimental analysis
[0056] Detection of cell proliferation by MTT (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide)
[0057] (1) Co...
Embodiment 2
[0082] Example 2 The effect of ferrous glycinate on the migration of RA-FLS in rheumatoid arthritis in vitro
[0083] 1. RA-FLS culture:
[0084] After resuscitation of RA-FLS, it was added to a 25T culture flask in DMEM medium containing 10% Australian fetal bovine serum (purchased from Thermo Fisher Scientific, USA), and placed at 37°C and 5% CO. 2 The cells were cultured in a constant temperature incubator, and the medium was replaced every 2 days. When the cell density reached about 95%, the cells were passaged, and the experiments were carried out within 3 to 10 passages.
[0085] 2. Scratch test:
[0086] After the cells reached an appropriate density (about 80% confluence), they were digested with trypsin for 2 minutes, and the digestion was terminated with complete medium (DMEM medium containing 10% Australian fetal bovine serum), centrifuged at 800 rpm for 5 minutes, and the supernatant was removed. After that, the cells were suspended in complete medium (DMEM mediu...
Embodiment 3
[0089] Example 3 The effect of ferrous glycinate on the secretion of inflammatory factors in M1-type macrophages induced by THP-1 in vitro
[0090] 1. THP-1 culture
[0091] After the THP-1 cells were recovered, they were placed in a 25T culture flask containing 10% fetal bovine serum in 1640 medium (purchased from Thermo Fisher Scientific, USA), at 37°C and 5% CO. 2 Culture in a constant temperature incubator, change the medium every 2 days, and cultivate to 8 × 10 5 Cells / mL can be passaged.
[0092] 2. Western blot experiment
[0093] (1) Collect the cell suspension of the above cultured THP-1 cells and inoculate them in a six-well plate at a density of 1×10 6 cells / well, 37°C and 5% CO 2 The experiment was carried out after culturing for 24 h under the conditions. The experiment was divided into six groups, namely blank control group, stimulation control group and drug-adding group of different concentrations of ferrous glycinate (4 groups), with 2 duplicate wells in ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More