Application of IL-4 in preparation of medicine for treating retinal degenerative diseases
A technology of retinal degeneration and IL-4, applied in the application field of drugs for the treatment of retinal degenerative diseases, to achieve the effect of reducing folds, reducing retinal exudation, and increasing cell layer thickness
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] Example 1 Construction of disease model
[0029] Sodium iodate (NaIO 3 ) is a stable oxidant that increases the conversion of glycine in melanocytes into glyoxylic acid, a cytotoxic compound, through a specific chemical reaction with melanin, so NaIO 3 RPE cells were selectively damaged, followed by secondary damage to photoreceptor cells, mimicking the pathological changes of retinal degeneration. Thereby the present invention adopts NaIO 3 Construction of related disease models. Specific steps are as follows:
[0030] (1) Dissolve IL-4 in phosphate buffered saline (PBS) to prepare a concentration of 2 μg / μL to obtain IL-4 solution for use;
[0031] (2) C57BL / 6J mice (purchased from Jiangsu Jicui Yaokang Biotechnology Co., Ltd.) at 8-12 weeks were randomly divided into three groups, 10 in each group;
[0032] (3) Group 1 (blank group) was injected with PBS into the tail vein and 1 μL of PBS into the vitreous cavity at the same time; Group 2 (model group) was injec...
Embodiment 2
[0034] Example 2 Electroretinogram (ERG) to evaluate the retinal function of mice after IL-4 treatment
[0035] The whole process of the experiment was carried out under the condition of weak red light illumination.
[0036] (1) After Example 1 NaIO 3 On the 7th day after modeling, the electroretinogram (ERG) of the above-mentioned groups of mice was detected using an electroretinograph (Celeris-Diagnosyssystem). Mice were dark-adapted for 12 hours before examination. Intraperitoneal injection of 4.3% chloral hydrate (10mL / kg) for anesthesia, and compound tropicamide eye drops to dilate the pupils;
[0037] (2) Fix the mouse on the experimental board and install the electrodes. Drop a little methylcellulose on the cornea, stick the recording electrode (ring-shaped corneal electrode made of 0.2mm copper wire) on the corneal rim, and place the reference electrode (needle electrode) on the same side of the mouse’s cheek Subcutaneously, the ground electrode was placed under th...
Embodiment 3
[0040] Example 3 Optical coherence tomography (OCT) evaluation of retinal thickness in mice treated with IL-4
[0041] (1) After Example 1 NaIO 3 On the seventh day after modeling, the mice in the above groups were detected by OCT using an electroretinograph (SPECTRALIS-OCT, Heidelberg, Germany). Before the examination, 4.3% chloral hydrate (10mL / kg) was injected intraperitoneally for anesthesia, and compound tropicamide eye drops were used to dilate the pupils;
[0042] (2) After anesthesia, fix the head of the mouse on the proper position of the OCT jaw frame, clamp the bulbar conjunctiva with tweezers to rotate the eyeball position, and align the OCT detection light source with the laser spot area. Use the three-dimensional scanning method set in the computer to scan the laser spots, take the point 3mm away from the optic nerve head as the center of the circle, and scan with a radius of 1.5mm. The scanning range covers the four directions of the retina: transverse, longit...
PUM
| Property | Measurement | Unit |
|---|---|---|
| concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More