Radix angelicae sinensis blood enriching decoction and application of effective component astragaloside thereof to preparation of medicine for treating alcoholic liver disease
A technology of alcoholic liver disease and astragaloside IV, applied in the field of medicine, can solve problems such as promoting hepatic steatosis and harmful inflammation
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Embodiment 1
[0041] Example 1 Effect of Danggui Buxue Decoction and Astragaloside IV on ALD Rat Liver Tissue Fat Metabolism
[0042] 1.1 Materials and methods
[0043] Preparation of Danggui Buxue Decoction: Weigh the crude drug according to the compatibility ratio of Astragalus and Angelica 5:1, add 10 times the amount of cold water to soak for 30 minutes, start decocting with strong fire, after boiling, decoct with slow fire for 40 minutes, filter while it is hot, and the filtrate will drip naturally do. Add 6 times the amount of water for the second decoction, the decoction method is the same as above, and combine the filtrate. Concentrate by rotary evaporation in a rotary evaporator at a constant temperature of 60°C to a concentration of 1g / m1, and store in a refrigerator at 4°C for later use.
[0044] Preparation of ALD rat model: 40 SPF grade male SD rats, weighing between 200±20g. Rats were randomly divided into blank group, model group, Danggui Buxue Decoction group, and astraga...
Embodiment 2
[0055] Example 2 Effects of Danggui Buxue Decoction and Astragaloside IV on liver tissue cell apoptosis, oxidative stress, and inflammatory pathways and factors in ALD rats
[0056] 2.1 Materials and methods
[0057] The same as the materials and methods under "1.1". The liver tissue preserved in liquid nitrogen was taken out and ground into powder. Operate according to the kit instructions and detect relevant indicators.
[0058] 2.2 Grouping
[0059] They were randomly divided into 7 groups: normal group, model group, Danggui Buxue Decoction group (3.5g / kg / d), astragaloside IV group (25μg / kg / d), 10 rats in each group.
[0060] 2.3 Detection and indicators
[0061] ① TUNEL staining to observe cell apoptosis in liver tissue.
[0062] ②ELISA: ROS, SOD.
[0063] ③RT-qPCR: TNF-α, IL-10; PPARγ.
[0064] ④Western blot: Bax, Bcl-2, ATP, CYP2E1, NF-κB.
[0065] 2.4 Results
[0066] The experimental results showed that: compared with the blank group, the rat liver tissue apopto...
Embodiment 3
[0068] Example 3 Effect of Astragaloside IV on Ethanol-Induced AML-12 Cell Lipid Metabolism, Inflammation and Oxidative Stress
[0069] 3.1 Materials and methods
[0070] AML-12 hepatocytes were cultured in a cell culture incubator at 37°C and 5% CO2 using a medium containing 10% fetal bovine serum. AML-12 cells were treated with 50, 75, 100, 150 or 200 mM ethanol and 50, 75, 100, 150 or 200 μg / mL astragaloside IV for 48 hours, respectively. Then, the cells were collected to analyze the cell viability by MTT method. Select 200mM ethanol and 200μg / mL astragaloside IV for subsequent determination. Untreated and ethanol-treated AML-12 cells were used as controls. The liver cell line AML-12 was purchased from the Type Culture Collection Center of the Chinese Academy of Sciences (Shanghai, China), and the cell culture reagents were purchased from Thermo Fisher Scientific (Massachusetts, USA).
[0071] 3.2 Grouping
[0072] Blank group, model group (200mM ethanol), astragalosid...
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