Application of bifidobacterium breve M-16V in medicine preparation
A technology of Bifidobacterium breve and medicine, applied in the field of medicine, can solve the problems of inability to defend, excessive attack, etc., and achieve the effect of improving the immunity of the body
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] An in vivo mouse model of immune disorder was established with polystyrene, and the therapeutic effect of Bifidobacterium breve M-16V on immune disorders was studied through the intervention of Bifidobacterium breve M-16V.
[0032] Two-month-old ICR mice were selected, and the model group was given 5 μm polystyrene 1000 μg / L by gavage for 4 weeks, six days a week. Treatment group: 2 × 10 8 CFU / g / d Bifidobacterium breve M-16V, the gavage time was the same as the model group; control group: PBS was gavage, the gavage time was the same as above.
[0033] One month after oral gavage, mice were euthanized and tissues were collected. For experimental purposes, the spleen was mechanically isolated into a single-cell suspension for further measurements. Feces and intestines were stored at -80°C for 16S rDNA gene sequencing, untargeted metabolomic detection and RT-PCR analysis, respectively.
[0034] Bifidobacterium breve M-16V can change the composition of intestinal flora a...
Embodiment 2
[0036] Changes in splenic lymphocyte subsets were determined by flow cytometry. Wash T cells with flow cytometry staining buffer, block T cells with Fc blocking solution, and stain at 4°C for 30 min. Resuspend after washing. 10 in 100 μL PBS 6 cells were added to a 5 mL fluorescence-activated cell sorting tube. T cell depletion / replenishment kinetics were analyzed by flow cytometry.
[0037] like figure 2 As shown, the immunotoxicity of PS may be directly attributed to the hyperfunction of antigen-specific Th2 cells and the marked decrease of Th1 cells, which leads to the occurrence of type 2 immunity. The role of Bifidobacterium breve M-16V in the differentiation of spleen lymphocytes was shown to inhibit the levels of Th2 and Th17 lymphocyte subsets. Dietary intervention with Bifidobacterium breve M-16V suppressed allergic symptoms and reduced Th2 activation in mice.
Embodiment 3
[0039] Total RNA was isolated from the gut using Trizol reagent. RNA was then used to synthesize cDNA using a reverse transcriptase kit. RT-PCR was performed using SYBR Green system.
[0040] Primer sequence:
[0041] MyD88, F: GTGAACTGGAAGATCTTCGAGA, as shown in SEQ ID NO.1;
[0042] R: ACTTGGTCAGTACTTTAGGTGG; as shown in SEQ ID NO.2.
[0043] MHC-II, F: GTGAACTGGAAGATCTTCGAGA, as shown in SEQ ID NO.3;
[0044] R: ACTTGGTCAGTACTTTAGGTGG; as shown in SEQ ID NO.4.
[0045] TRIF, F: ACGATCCTGCTCCTGACTGCTAG, as shown in SEQ ID NO.5;
[0046] R:TCCTGCCTCCCAGACTGTGTAAG; as shown in SEQ ID NO.6.
[0047] GAPDH transcript levels were identified as a housekeeping gene. RT-PCR was performed as follows: denaturation for 10 min at 95°C, followed by 40 cycles of 10s at 95°C and 30s at 60°C. use 2 -ΔΔCt The relative quantification of gene expression was calculated by the method.
[0048] like image 3The results of RT-PCR detection of the expression of key genes in the small int...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More - R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com