Method for counting number of endometrial developed embryos of caenorhabditis elegans

Abstract

The invention relates to a method for counting the number of endometrial developed embryos of caenorhabditis elegans. According to the method, caenorhabditis elegans is taken as a model organism, and a whole set of experimental process is designed to detect and count the number of embryos in the uterus of the caenorhabditis elegans. According to the method disclosed by the invention, nematode bodies can be transparent, and embryos which are inconvenient to count in pregnant insect bodies can be completely exposed, so that the number of the embryos can be conveniently counted under a common optical stereoscopic microscope. The method is suitable for caenorhabditis elegans which are exposed from the L1 stage and are in the pregnancy stage in the final state. According to the method, the experimental method which is easy to observe, rapid, simple, convenient, accurate and reliable can be provided for researching the reproductive capacity of caenorhabditis elegans, especially toxicological and pharmacological researches taking the number of embryos in uterus as an index, the detection result has high accuracy and reliability, and the method is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for counting the number of embryos developed in the uterus of Caenorhabditis elegans. Background technique [0002] Caenorhabditis elegans, a non-parasitic nematode, is an ideal model organism for research due to its easy culture, translucent body, short life cycle, and complete genome sequencing database. Most C. elegans are hermaphrodites with complete reproductive structure, including two gonad arms, two seminal vesicles, and a uterus. They can reproduce by self-fertilization and are a classic model for the study of reproductive effects in biomedicine, environmental toxicology and other disciplines. . Under normal circumstances, the physiological research indicators used to study the reproduction of C. elegans include gonad apoptosis, genital morphology, the number of embryos developing in the uterus, the morphology and number of oocytes, the morphology and n...

AI Technical Summary

Problems solved by technology

[0003] Because the structure of Caenorhabditis elegans during pregnancy has fully developed gonads, uterus, intestines, etc., which cover some embryos in the nematode, the direct observation of the number of embryos in the pregnant worm is not accurate enough

In addition, because Caenorhabditis elegans crawls faster under the microscope, it is difficult to clearly observe the shape and number of embryos developing in the uterus of the nematode, and it is impossible to accurately count the number of embryos in the uterus of each nematode, resulting in large errors in the evaluation of reproductive effects , the result is not accurate enough

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