Compound, preparation method and application thereof, and microorganism for producing compound
A technology of compounds and microorganisms, applied in the field of compounds
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Embodiment 1
[0078] This example is used to illustrate the acquisition of Bacillus licheniformis provided by the present invention
[0079] 1.1 Enrichment and isolation of hot spring thermophiles
[0080] Five main hot springs (N104°35′09.9”N, E31°58′83.6”E) were collected in Anxian Bioreef National Geopark, Mianyang, southwest China, and hot spring water samples were collected using sterile containers. The sample is 2m away from the shore and the depth is 40cm. away from the edge to be a representative sample. The samples were stored at 4 °C and transported to the laboratory. After measuring the physical and chemical parameters such as hot spring salinity and pH in the laboratory, the samples were filtered through a 0.45 mm membrane, and the filter membrane was placed in tryptic soy broth (TSB) for incubation at 50 °C-90 °C. A gradient was set at each 10°C for a total of 5 groups, and the shaking speed was 160 r / min and incubated for 3 d. Dilute 1 mL of enriched solution with sterile w...
Embodiment 2
[0098] This example is used to illustrate the preparation of strain QX8 crude extract
[0099] The basic fermentation medium was inoculated with activated Bacillus licheniformis QX8, incubated at 55°C on a shaker for 72 hours, and centrifuged at 12,000 r / min for 10 minutes to obtain a cell-free supernatant. The supernatant was mixed with an equal volume of ethyl acetate and incubated overnight at 4°C in a separate extraction tank. The ethyl acetate was then evaporated in a rotary evaporator, and the remaining residue was concentrated in a vacuum concentrator and lyophilized by a lyophilizer to obtain a crude extract.
Embodiment 3
[0101] This example is used to illustrate the performance of the screened strain QX8
[0102] 3.1 Evaluation of antibacterial activity
[0103] Dilute Pseudomonas aeruginosa, Salmonella Enteritidis, Clostridium, Staphylococcus aureus, Escherichia coli to OD 600 = 0.1, add each type of bacteria to a 96-well plate (one row of wells per bacteria), add 20 mg / mL crude extract (dissolved in distilled water) to the first well of each row and dilute each well successively, and cultivate at 37°C for 24 hours for observation The growth of bacteria was repeated three times for each bacteria. The result is as image 3 shown.
[0104] Depend on image 3 It can be seen that the QX8 medium is extracted with ethyl acetate and the extract after washing with water has a broad-spectrum antibacterial activity, which can significantly inhibit the growth of Pseudomonas aeruginosa, Escherichia coli, Salmonella enteritidis, and Staphylococcus aureus.
[0105] 3.2 Stability assessment of antibact...
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