Probiotic powder containing human body homologous strains and application thereof
A technology of probiotics and bacterial strains, applied in the field of microorganisms, can solve problems such as allergies, lactose intolerance, and intestinal flora imbalance, and achieve the effects of eliminating toxins, health and side effects, and improving lactose intolerance
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Embodiment 1
[0051] Example 1: Preparation of Bifidobacterium bifidum BGN4 freeze-dried bacterial powder
[0052] 1) Bifidobacterium bifidum BGN4 was streaked on MRS solid medium and cultured at 37°C for 48 hours to obtain a single colony.
[0053] 2) Picking a single colony and inoculating it into MRS liquid medium, culturing for 24-48 hours at 37° C. for activation, and obtaining a first-class seed liquid.
[0054] 3) Transfer the first-grade seed liquid to the seed medium according to the inoculum amount of 3% (v / v) to obtain the second-grade seed liquid. The culture condition is 37° C. for 24-48 hours.
[0055] 4) The secondary seed liquid is respectively inserted into the fermentation medium for high-density fermentation, the inoculum amount is 3% (v / v), the culture condition is 35-37° C., and the culture is cultured for 24-48 hours.
[0056] 5) The bacterial density in the fermented liquid reaches 10 8 ~10 9 CFU / mL, the fermentation broth was collected separately; the fermentation...
Embodiment 2
[0059] The preparation of embodiment 2 Bifidobacterium longum BORI freeze-dried bacteria powder
[0060] 1) Bifidobacterium longum BORI was streaked on MRS solid medium and cultured at 37°C for 48h to obtain a single colony.
[0061] 2) Picking a single colony and inoculating it into MRS liquid medium, culturing for 24-48 hours at 37° C. for activation, and obtaining a first-class seed liquid.
[0062] 3) Transfer the first-grade seed liquid to the seed medium according to the inoculum amount of 3% (v / v) to obtain the second-grade seed liquid. The culture condition is 37° C. for 24-48 hours.
[0063] 4) The secondary seed liquid is respectively inserted into the fermentation medium for high-density fermentation, the inoculum amount is 3% (v / v), the culture condition is 35-37° C., and the culture is cultured for 24-48 hours.
[0064] 5) The bacterial density in the fermented liquid reaches 10 8 ~10 9 CFU / mL, the fermentation broth was collected separately; the fermentation b...
Embodiment 3
[0067] The preparation of embodiment 3 Bifidobacterium lactis AD011 freeze-dried bacteria powder
[0068] 1) Bifidobacterium lactis AD011 was streaked on MRS solid medium and cultured at 37°C for 48h to obtain a single colony.
[0069] 2) Picking a single colony and inoculating it into MRS liquid medium, culturing for 24-48 hours at 37° C. for activation, and obtaining a first-class seed liquid.
[0070] 3) Transfer the first-grade seed liquid to the seed medium according to the inoculum amount of 3% (v / v) to obtain the second-grade seed liquid. The culture condition is 37° C. for 24-48 hours.
[0071] 4) The secondary seed liquid is respectively inserted into the fermentation medium for high-density fermentation, the inoculum amount is 3% (v / v), the culture condition is 35-37° C., and the culture is cultured for 24-48 hours.
[0072] 5) The bacterial density in the fermented liquid reaches 10 8 ~10 9 CFU / mL, the fermentation broth was collected separately; the fermentation...
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