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Probiotic powder containing human body homologous strains and application thereof

A technology of probiotics and bacterial strains, applied in the field of microorganisms, can solve problems such as allergies, lactose intolerance, and intestinal flora imbalance, and achieve the effects of eliminating toxins, health and side effects, and improving lactose intolerance

Pending Publication Date: 2022-08-02
比菲德(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the characteristics of intestinal flora imbalance in infants and children, such as milk flap, flatulence, constipation and diarrhea, allergies, lactose intolerance and other basic intestinal discomfort, according to the existing clinical and research findings, Proposed a probiotic powder containing human homologous strains and its application, specifically as follows:

Method used

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  • Probiotic powder containing human body homologous strains and application thereof
  • Probiotic powder containing human body homologous strains and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Preparation of Bifidobacterium bifidum BGN4 freeze-dried bacterial powder

[0052] 1) Bifidobacterium bifidum BGN4 was streaked on MRS solid medium and cultured at 37°C for 48 hours to obtain a single colony.

[0053] 2) Picking a single colony and inoculating it into MRS liquid medium, culturing for 24-48 hours at 37° C. for activation, and obtaining a first-class seed liquid.

[0054] 3) Transfer the first-grade seed liquid to the seed medium according to the inoculum amount of 3% (v / v) to obtain the second-grade seed liquid. The culture condition is 37° C. for 24-48 hours.

[0055] 4) The secondary seed liquid is respectively inserted into the fermentation medium for high-density fermentation, the inoculum amount is 3% (v / v), the culture condition is 35-37° C., and the culture is cultured for 24-48 hours.

[0056] 5) The bacterial density in the fermented liquid reaches 10 8 ~10 9 CFU / mL, the fermentation broth was collected separately; the fermentation...

Embodiment 2

[0059] The preparation of embodiment 2 Bifidobacterium longum BORI freeze-dried bacteria powder

[0060] 1) Bifidobacterium longum BORI was streaked on MRS solid medium and cultured at 37°C for 48h to obtain a single colony.

[0061] 2) Picking a single colony and inoculating it into MRS liquid medium, culturing for 24-48 hours at 37° C. for activation, and obtaining a first-class seed liquid.

[0062] 3) Transfer the first-grade seed liquid to the seed medium according to the inoculum amount of 3% (v / v) to obtain the second-grade seed liquid. The culture condition is 37° C. for 24-48 hours.

[0063] 4) The secondary seed liquid is respectively inserted into the fermentation medium for high-density fermentation, the inoculum amount is 3% (v / v), the culture condition is 35-37° C., and the culture is cultured for 24-48 hours.

[0064] 5) The bacterial density in the fermented liquid reaches 10 8 ~10 9 CFU / mL, the fermentation broth was collected separately; the fermentation b...

Embodiment 3

[0067] The preparation of embodiment 3 Bifidobacterium lactis AD011 freeze-dried bacteria powder

[0068] 1) Bifidobacterium lactis AD011 was streaked on MRS solid medium and cultured at 37°C for 48h to obtain a single colony.

[0069] 2) Picking a single colony and inoculating it into MRS liquid medium, culturing for 24-48 hours at 37° C. for activation, and obtaining a first-class seed liquid.

[0070] 3) Transfer the first-grade seed liquid to the seed medium according to the inoculum amount of 3% (v / v) to obtain the second-grade seed liquid. The culture condition is 37° C. for 24-48 hours.

[0071] 4) The secondary seed liquid is respectively inserted into the fermentation medium for high-density fermentation, the inoculum amount is 3% (v / v), the culture condition is 35-37° C., and the culture is cultured for 24-48 hours.

[0072] 5) The bacterial density in the fermented liquid reaches 10 8 ~10 9 CFU / mL, the fermentation broth was collected separately; the fermentation...

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Abstract

The invention discloses probiotic powder containing human body homologous strains and application of the probiotic powder, and belongs to the technical field of microorganisms and the technical field of medicines. The probiotic powder is composed of bifidobacterium bifidum BGN4, bifidobacterium longum BORI, bifidobacterium lactis AD011, lactobacillus rhamnosus BH09, dual prebiotics and 13 basic nutrients, can regulate intestinal flora disorder of children, and is specifically embodied in that the probiotic powder can improve the intestinal micro-ecological environment, prevent rotavirus infection, improve the immunity of children, and improve the immunity of children. The composition can prevent and relieve children eczema and allergic dermatitis, relieve diarrhea, constipation and other intestinal problems, improve lactose intolerance and effectively eliminate toxins in food. Therefore, the probiotic powder containing the bifidobacterium bifidum BGN4, the bifidobacterium longum BORI, the bifidobacterium lactis AD011 and the lactobacillus rhamnosus BH09 has a great application prospect in regulating the intestinal flora disorder of the children.

Description

technical field [0001] The invention relates to a probiotic powder containing human homologous strains and its application, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] The establishment and composition of the intestinal flora in early childhood can be traced back to the establishment of the intestinal flora in the mother's womb during infancy and childhood. The structure of the maternal flora transmitted through the placenta promotes fetal immunity and intestinal mucosal development. [0003] Intestinal microflora is the core issue of human microecology researchers. With the deepening of our understanding of the microecology and the wide application of molecular biology techniques, it has been confirmed that the intestinal flora plays a decisive role in the important physiological functions of children, such as the development and maturation of immunity, metabolism, nutrition, etc. Diseases, intestina...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61K9/14A61P1/00A61K31/07A61K31/355A61K31/375A61K31/4415A61K31/455A61K31/51A61K31/519A61K31/525A61K31/59A61K31/702A61K31/714A61K33/06A61K33/26A61K33/30A61K35/745
CPCA61K35/745A61K35/747A61K33/06A61K33/26A61K33/30A61K31/07A61K31/59A61K31/355A61K31/51A61K31/525A61K31/4415A61K31/714A61K31/375A61K31/455A61K31/519A61K31/702A61K9/14A61P1/00A61K2300/00Y02A50/30
Inventor 金海鹰
Owner 比菲德(北京)生物科技有限公司
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