Multicolor light-emitting nano material for biomedical dynamic imaging and preparation method of multicolor light-emitting nano material
A technology of dynamic imaging and nanomaterials, applied in the field of nanomaterials, to achieve the effects of no cytotoxicity and photobleaching, easy control of reaction conditions, and good biocompatibility
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Embodiment 1
[0042] Example 1 Screening of raw materials
[0043] Prepare HAuCl according to Table 1 below 4 . 3H 2 O was experimented with BSA raw material.
[0044] Table 1
[0045]
[0046]
[0047] The experimental steps are as follows:
[0048] 1.1. Combine the BSA to be reacted with HAuCl 4 . 3H 2 O is formulated into a mother liquor of a certain concentration, and groups 1-13 are respectively added with BSA and HAuCl according to the proportions in Table 1 4 . 3H 2O in a 25mL single-neck bottle, stir well, and mix evenly to obtain a complex of BSA and Au; the total volume of the reaction solution is 1–8 mL, and the stirring rate is 800–1500 r / min;
[0049] 1.2. Add an appropriate amount of NaOH aqueous solution to the reaction flask, adjust the pH value of the reaction to 10, control the reaction conditions in the air atmosphere, observe the color change, increase the reaction temperature and continue the reaction for a period of time to obtain a crude product. A co...
Embodiment 2
[0054] Example 2 PH value screening
[0055] experimental method
[0056] 2.1. BSA and HAuCl to be reacted 4 . 3H 2 O is formulated into a mother liquor of a certain concentration, and BSA and HAuCl are added. 4 . 3H 2 O (according to the proportion of group 9) in a 25mL single-neck bottle, fully stirred, and mixed evenly to obtain a complex of BSA and Au; the total volume of the reaction solution was 1–8 mL, and the stirring rate was 800–1500 r / min.
[0057] 2.2. Add an appropriate amount of NaOH aqueous solution to the reaction flask, adjust the pH value of the reaction (adjust the pH value as shown in Table 2), control the reaction conditions in the air atmosphere, observe the color change, increase the reaction temperature and continue the reaction for a period of time to obtain Crude product; a color change was observed, ie a change in the color of the reaction solution from pale yellow to dark red. Increase the reaction temperature, adjust the temperature of the r...
Embodiment 3
[0064] Example 3 Au(I)-BSA nanoparticles
[0065] experimental method:
[0066] For the detection of dynamic light scattering, 30 μL of the original solution of Au(I)-BSA nanoparticles prepared in Group 22 in Example 2 was diluted to 3 mL, and then placed in a dynamic light scattering instrument to measure its hydrated particle size in an aqueous solution .
[0067] test results:
[0068] Dynamic Light Scattering (DLS) map by Au(I)-BSA nanoparticles ( image 3 ) analysis, it is concluded that the hydrated particle size of the synthesized material is about 30.6 nm, which is no different from the statistical result of TEM. In addition, it is proved that the nanoparticles have good dispersibility in aqueous solution, and the dispersion coefficient PDI=0.17, which is suitable for cell and biological imaging.
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