Establishment of zebra fish osteoporosis diagnostic standard

A standard technique for the diagnosis of osteoporosis, applied in the field of biomedicine, can solve problems such as time-consuming, no prompts, complicated staining procedures, etc., and achieve the effect of simple operation, accurate and reliable results, and fast imaging speed

Pending Publication Date: 2022-08-02
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods for constructing zebrafish osteoporosis models in the prior art all show that zebrafish has a significant reduction in bone mass, but it does not indicate which part and how much bone mass is reduced to reach the standard of osteoporosis; and The models used in the prior art are usually zebrafish juveniles, and clinically osteoporosis is more common in middle-aged and elderly people, and the use of osteoporotic juvenile fish models cannot simulate the osteoporosis model well; it uses bone The dyeing procedure of dyeing is complicated and time-consuming

Method used

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  • Establishment of zebra fish osteoporosis diagnostic standard
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  • Establishment of zebra fish osteoporosis diagnostic standard

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Establishment of a diagnostic method for zebrafish osteoporosis

[0042] 1. Configuration of the solution

[0043] Prednisolone solution: Weigh 3.6 g of prednisolone, dissolve it in 10 mL of dimethyl sulfoxide (DMSO), dissolve it fully, dilute to 1 L of distilled water, and store in a sealed container with a concentration of 10 mmol / L. Dilute to 150 μmol / L before use.

[0044] 0.03% MS222 (ethyl m-aminobenzoate methanesulfonate): Weigh 30 mg of MS222, dissolve in 100 mL of distilled water, seal and store at 4°C.

[0045] 2. Establishment of zebrafish osteoporosis criteria

[0046] (1) Select 12 adult female zebrafish with normal development from 3 to 5 months and divide them into two groups. One group is placed in culture water as normal group, and the other group is placed in 150μmol / L prednisolone for induction and fed once a day. The brine shrimp were replaced with fresh induction solution every 2 days; after 21 days of feeding, they were euthanized in 0...

Embodiment 2

[0060] Example 2 Drug evaluation of zebrafish osteoporosis model

[0061] (1) Osteoporosis model group: 12 normal adult female zebrafish from 3 to 5 months were selected and divided into two groups. One group was induced with 150 μmol / L prednisolone, fed with common feed once a day, and changed every 2 days. One fresh induction solution; one group was fed a diet containing 200 μg / g FA, and the fresh induction solution was changed every two days;

[0062] After induction and feeding for 21 d, they were euthanized in 0.03% MS222, washed twice and fixed in 4% paraformaldehyde for 24-48 h.

[0063] (2) Cut out the caudal body segment of the zebrafish and store it in 70% alcohol.

[0064] (3) Micro-CT scanning: Scan the sagittal plane of the caudal body of zebrafish with SCANO MEDICALμCT 100, select the caudal body C1-C4 for three-dimensional reconstruction, obtain the bone mineral density (BMD), and record.

[0065] Scanning parameters are as follows: X-ray source is 55kV, 200μA...

Embodiment 3

[0069] Example 3 Drug evaluation of zebrafish osteoporosis model

[0070] (1) Osteoporosis model group: 12 normal adult female zebrafish from 3 to 5 months were selected and divided into two groups. One group was induced with 150 μmol / L prednisolone, fed with common feed once a day, and changed every 2 days. One fresh induction solution; one group was fed a diet containing 2 mg / g FA, and the fresh induction solution was changed every two days;

[0071] After induction and feeding for 21 d, they were euthanized in 0.03% MS222, washed twice and fixed in 4% paraformaldehyde for 24-48 h.

[0072] (2) Cut out the caudal body segment of the zebrafish and store it in 70% alcohol.

[0073] (3) Micro-CT scanning: Scan the sagittal plane of the caudal body of zebrafish with SCANO MEDICALμCT 100, select the caudal body C1-C4 for three-dimensional reconstruction, obtain the bone mineral density (BMD), and record.

[0074] Scanning parameters are as follows: X-ray source is 55kV, 200μA, ...

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Abstract

The invention discloses an establishment method of zebra fish osteoporosis diagnosis standards and a zebra fish osteoporosis diagnosis system. The method comprises the following steps: constructing a zebra fish osteoporosis model, scanning a zebra fish skull and/or spine by using Micro-CT, counting values of zebra fish bone density and bone volume percentage, calculating Mean +/-SD, calculating a T value through a Mean value and an SD value, judging, and further considering a bone mass loss value of a scanned part according to the bone density and/or bone volume percentage, so as to determine the osteoporosis of the zebra fish. The zebra fish osteoporosis diagnosis standard is obtained. The zebra fish osteoporosis diagnostic standard constructed by the invention can be directly used for evaluating medicines, and provides support for subsequent judgment of the degree of relieving bone loss by medicines. Diagnostic data support is provided for establishment of a zebrafish osteoporosis model, development of osteoporosis drugs and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the establishment of diagnostic criteria for zebrafish osteoporosis. Background technique [0002] Osteoporosis (OP) is a common bone disease characterized by bone loss, degeneration of bone tissue and destruction of bone microstructure due to various reasons, resulting in increased brittle bone and a system prone to fractures Bone disease. Osteoporosis is divided into two categories: primary and secondary. Among them, primary osteoporosis is further divided into postmenopausal osteoporosis (type I) and senile osteoporosis (type II). and idiopathic osteoporosis (including juvenile type). The clinically used dual energy X-ray absorptiometry (DXA) measurement value is currently the gold standard for the diagnosis of osteoporosis in the world. The clinically recommended measurement sites are lumbar spine 1-4, total hip and femoral neck. , and calculate the T ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16H50/20G16H50/50
CPCG16H50/20G16H50/50Y02A40/81
Inventor 王佳力高楚丹
Owner SUN YAT SEN UNIV
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