Diphenyl ether phenol as well as preparation method and application thereof

A technique for culturing diphenyl ether and strains, applied in the fields of botany equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as diphenyl ether phenolic compounds that have not been seen yet, and achieve the effect of broad application prospects

Active Publication Date: 2022-08-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there have been no reports on the use of natural diphenyl ether phenolic compounds with important antibacterial activity from endophytic fungi derived from mangrove plants as fungicides for plant pathogenic fungi and antibacterial agents for chicken Escherichia coli

Method used

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  • Diphenyl ether phenol as well as preparation method and application thereof
  • Diphenyl ether phenol as well as preparation method and application thereof
  • Diphenyl ether phenol as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The diphenyl ether phenol Phomaspether H was prepared by the following steps:

[0046] (1) First, in the bacterial culture medium, the endophytic fungus Phoma sp. L28 of Myoporum bontioides A.Gray is cultured, and the used bacterial culture medium is a medium containing 1.0% glucose (weight). percentage, the same below), yeast extract 0.01%, peptone 0.01, agar 2.0%, sodium chloride 0.2%, and the rest are water. When in use, it is made into a test tube slant, and the above strains are cultured at 28°C for 5 days;

[0047] (2) Phoma sp. L28 is then fermented and cultured, and the fermentation medium used is 40% (weight percent, the same below) of japonica rice, and the remainder is water, and is cultured at room temperature at 20° C. for 60 days.

[0048](3) extracting the fermented strain in step (2) with ethyl acetate 3 times, concentrating the extract under reduced pressure, chromatographic separation in a 200-300 mesh silica gel column, and eluting with petroleum eth...

Embodiment 2

[0050] The diphenyl ether phenol Phomaspether H was prepared by the following steps:

[0051] (1) First, the endophytic fungus Phoma sp. L28 of Myoporum bontioides A.Gray was cultured in the culture medium, and the culture medium used was 3.0% glucose, yeast Paste 1.5%, peptone 1.5%, agar 2.5%, sodium chloride 2.0%, and the rest is water. When in use, it is made into a test tube slant, and the above strains are cultured at 20°C for 10 days;

[0052] (2) carry out fermentation culture to Phoma sp. L28 again, and the fermentation medium used is 50% of japonica rice, and the balance is water; at 30° C., stand and cultivate for 30 days;

[0053] (3) extracting the fermented strain in step (2) with ethyl acetate for 5 times, concentrating the extract under reduced pressure, chromatographic separation in a 200-300 mesh silica gel column, and eluting with a petroleum ether-ethyl acetate mixed solvent, The volume percentage of ethyl acetate was gradually increased from 0 to 75%; the...

Embodiment 3

[0055] The diphenyl ether phenol Phomaspether H was prepared by the following steps:

[0056] (1) First, the endophytic fungus Phoma sp. L28 of Myoporum bontioides A.Gray was cultured in the culture medium, and the culture medium used was a medium containing 2.0% glucose, yeast Paste 1.0%, peptone 1.0%, agar 2.0%, sodium chloride 1.0%, and the rest is water. When in use, it is made into a test tube slant, and the above strains are cultured at 25°C for 7 days;

[0057] (2) carry out fermentation culture to Phoma sp. L28 again, the fermentation medium used is 50% of japonica rice, and the balance is water; at 25 ℃, stand for 45 days;

[0058] (3) extracting the fermented strain in step (2) with n-butanol for 5 times, concentrating the extract under reduced pressure, chromatographic separation in a 200-300 mesh silica gel column, and eluting with a petroleum ether-ethyl acetate mixed solvent, The volume percentage of ethyl acetate was gradually increased from 0 to 75%; the elue...

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Abstract

The invention discloses diphenyl ether phenol as well as a preparation method and application thereof. The diphenyl ether phenol is characterized in that the molecular formula of the diphenyl ether phenol is C15H16O5, and the structural formula of the diphenyl ether phenol is shown as a formula I, the preparation method of the diphenyl ether phenol comprises the following steps: (1) carrying out strain culture on phoma sp. L28 in a strain culture medium; and (2) carrying out fermentation culture on the strain obtained in the step (1) in a fermentation culture medium, then extracting with an organic solvent, and carrying out vacuum concentration to obtain the diphenyl ether phenol. According to the present invention, the obtained diphenyl ether phenol has strong inhibition effects on banana colletotrichum gloeosporioides, tomato fusarium oxysporum and O78 serotype Escherichia coli, and can be used as the related microbial agricultural bactericide or veterinary drug preparation.

Description

technical field [0001] The invention belongs to the field of biochemical industry, in particular to a diphenyl ether phenol and a preparation method and application thereof. Background technique [0002] According to statistics, the direct economic losses to agriculture caused by plant fungal diseases each year are as high as hundreds of billions of dollars. However, the resistance of existing agricultural antibiotics is becoming more and more serious, and it is urgent to find efficient and safe agricultural fungicides to maintain high and stable crop yields and prevent agricultural products from being infected by plant fungal diseases during storage. [0003] Mangrove endophytes are the second largest group of marine fungi. Due to their different living environments, they have unique metabolic patterns that are different from those of terrestrial fungi and can produce novel biologically active substances. However, so far, there is no report on the use of natural diphenyl e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C43/295A01N31/16A01P3/00A01P1/00A61P31/04A61P31/10C12P7/22C12R1/645
CPCC07C43/295A01N31/16A61P31/04A61P31/10C12P7/22
Inventor 丁唯嘉李春远熊亚红祝钧杰陈若兰
Owner SOUTH CHINA AGRI UNIV
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