Preparation method of specific biological extraction protein-gamma protein

Abstract

The invention relates to the technical field of biological medicines, and particularly discloses a preparation method of a specific biologically extracted protein-gamma protein, and the preparation method of the specific biologically extracted protein-gamma protein comprises the following steps: pretreating a rotavirus antigen with a treating fluid to obtain a treated antigen; adding an adjuvant into the treated antigen, and fully emulsifying to obtain an immune vaccine; the immune vaccine is used for chicken immunization inoculation, and immunized eggs are collected; the preparation method comprises the following steps: immunizing an egg, carrying out egg yolk segmentation on the immunized egg to obtain egg yolk, uniformly stirring and dispersing the egg yolk, a phosphoric acid solution and polyethylene glycol, standing, centrifuging, collecting supernate, dialyzing and purifying the supernate through a semipermeable membrane, and carrying out spray drying to obtain the required specific biological extraction protein-gamma protein. The gamma protein prepared by the method is high in purity, good in stability, high in specificity and sensitivity, capable of being used for preparing oral specific gamma protein antibodies, small in immune interference, simple in preparation method, low in preparation cost and capable of being widely applied.

Description

technical field [0001] The present application relates to the technical field of biomedicine, and more particularly, to a method for preparing a specific biologically extracted protein-γ protein. Background technique [0002] Rotavirus (RV for short) is a double-stranded RNA virus belonging to the family Reoviridae. Rotavirus is the first pathogen that causes severe diarrhea in children under 5 years old in the world. Severe rotavirus enteritis mainly occurs in infants and young children aged 2 months to 3 years. Healthy body. Rotavirus infection route is the fecal-oral route, mainly infecting small intestinal epithelial cells, resulting in cell damage and diarrhea. There are a total of seven rotaviruses, which are numbered A, B, C, D, E, F and G in English letters. Among them, three groups A, B, and C can infect humans, and group A is the most common one. More than 90% of the cases of human rotavirus infection are also caused by group A. [0003] Once rotavirus enteriti...

AI Technical Summary

Problems solved by technology

[0005] With the deepening of research and application in the field of IgY, the application prospect of IgY is becoming wider and wider. Studies have shown that oral specific IgY antibodies have resistance to bovine and human rotaviruses, but the existing oral specific IgY antibodies Antibodies have limited resistance to bovine and human rotaviruses, and cannot effectively prevent and treat rotavirus enteritis

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