Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of RBBP7 gene/protein as drug target in preparation of products for diagnosing and treating male infertility diseases

A male infertility, protein technology, applied in the field of biomedicine

Pending Publication Date: 2022-08-05
ZHEJIANG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a lot of progress has been made in the research of RBBP7, there has been no report of male infertility caused by RBBP7 mutations clinically.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of RBBP7 gene/protein as drug target in preparation of products for diagnosing and treating male infertility diseases
  • Application of RBBP7 gene/protein as drug target in preparation of products for diagnosing and treating male infertility diseases
  • Application of RBBP7 gene/protein as drug target in preparation of products for diagnosing and treating male infertility diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening and identification of pathogenic genes in a non-obstructive azoospermia family

[0029] This study was approved by the Ethics Committee of the Obstetrics and Gynecology Hospital Affiliated to Zhejiang University School of Medicine, and informed consent was obtained from the patients. A non-obstructive azoospermia family was found in the Obstetrics and Gynecology Hospital affiliated to Zhejiang University School of Medicine ( figure 1 A), the 2 siblings of the family are both NOA patients, and their pathogenic causes exclude patients with organic diseases of the reproductive system, chromosomal abnormalities, AZF abnormalities, cryptorchidism, or mumps. Therefore, 0.2 ml of the idiopathic NOA patient's blood was collected, and the TIANamp Genomic DNA Kit was used to extract the DNA in the patient's blood (according to the instructions—extracting genomic DNA from whole blood).

[0030] Whole exome sequencing was performed on the DNA samples of the fam...

Embodiment 2

[0035] Example 2. The situation of 2 NOA patients in the family with RBBP7 mutation

[0036] H&E staining: After dewaxing and hydration, the paraffin sections of testicular puncture tissue were stained with hematoxylin staining solution for 5 minutes. The excess staining solution on the slides was washed with water, and then 0.5-1wt% hydrochloric acid alcohol (prepared with 70vol% alcohol) was used for color separation of 10. Second, rinsed with running water for 15-30 minutes, the nuclei turned blue after a short period of alkalization in lithium carbonate saturated solution, and briefly washed with distilled water; stained with 0.1-0.5wt% eosin for 1-5 minutes, and then used gradient ethanol Dehydration (70vol% ethanol for 2s→80vol% ethanol for 2s→90vol% ethanol for 2s→95vol% ethanol for 5min×2 times→anhydrous ethanol for 5min×2 times); clear with xylene, and finally seal the film with neutral resin, and the optical Observe and photograph under the microscope ( figure 2 A)...

Embodiment 3

[0040] Example 3 RBBP7 gene knockdown results in restricted proliferation of spermatogonia and spermatocytes, cell cycle arrest and increased apoptosis

[0041] Using siRNA interference technology (siRNA sequence 5'-CCAUGAAGGAGAAGUGAAUTT-3' (SEQ ID NO. 5); control sequence: 5'-AUUCACUUCUCCUCUUCAUGGTT-3' (SEQ ID NO. 6)), knock down GC-1 and GC-2 The mRNA expression of endogenous RBBP7 in cells, the results showed that the RNA and protein levels of RBBP7 were significantly down-regulated compared with the control group ( image 3 A-D). The growth curve of GC-1 and GC-2 cells with RBBP7 knockdown was analyzed by CCK8, and it was found that the proliferation ability of knockdown GC-1 and GC-2 cells was lower than that of the control group ( image 3 E, F).

[0042] The apoptosis of GC-1 and GC-2 cells with RBBP7 knockdown was analyzed by flow cytometry. The results showed that the apoptosis rate of GC-1 and GC-2 cells in RBBP7 knockdown was significantly higher than that in the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of an RBBP7 gene / protein as a drug target in preparation of a product for diagnosing and treating male infertility diseases. Experiments show that the RBBP7 mutant gene / protein causes male infertility, spermatogenesis disorder, spermatogenesis arrest and no sperm. The RBBP7 mutant gene / protein and one pathogenic mutation site of the RBBP7 mutant gene / protein can be used as a target gene for diagnosing male infertility. Meanwhile, the expression level of the RBBP7 of the RBBP7 gene / protein is remarkably reduced in non-obstructive azoospermia patients, and male infertility can be prevented and / or treated by improving the activity and / or expression quantity of the RBBP7 protein.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to the application of RBBP7 gene and / or protein as molecular markers in diagnosing male infertility diseases, and a method for detecting pathogenic mutation sites of human male infertility genes. Background technique [0002] In recent decades, male fertility around the world has been declining, and both the quantity and quality of sperm have declined. The prevalence of infertility among couples of childbearing age is as high as 15%, of which male factors account for about 50%. Male infertility has brought a heavy burden to individuals, families and society. Clinically, male infertility is mainly manifested as oligoasthenospermia or azoospermia, among which non-obstructive azoospermia (NOA), the lesion is mainly in the testis, resulting in no sperms in the semen. One of the worst cases of male infertility. The causes of male infertility are complex, including reproductive system anatomi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11G01N33/573
CPCC12Q1/6883G01N33/573C12Q2600/156C12Q2600/158C12Q2600/178C12Q2600/136G01N2800/367G01N2333/91057G01N2500/04
Inventor 李景平席咏梅郑慧梅金帆李晨杨小航张峰彬梁忠炎吴敬根侯佳汝
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com