Biochip analysis instrument

Abstract

The invention adopts the light emitted from two different wavelength light sources, and the light, respectively through the reflector, incomes to the vibrating mirror which swings back and force around the axis, then the angle-changing light reflected from the vibrating mirror is converted into the linear displacement light by the scanning object lens, then the linear displacement light is turned round to the biochip by the prism, which realizes one-dimensional scan of the biochip, and the other one is the motor-driving mechanical scan. The fluorescence emitted from the biochip is turned round to the scanning object lens by the prism, and it also passes through the vibrating mirror, the reflector and the focusing lens, then it, through the confocal-light diaphragm aperture, arrive at the filtering piece, and after the stray light is filtered out, it is converted into the electric signal by the light source.

Description

technical field [0001] The invention relates to a biochip analyzer. Background technique [0002] Biochips are widely used in gene research, drug research, disease diagnosis and other fields. It uses the principle of molecular hybridization to label the samples to be detected with fluorescent molecules, and then performs hybridization reactions with biochips of known structures, and uses biochip analyzers to detect the occurrence of hybridization. The fluorescent signal at the reaction site is displayed graphically. [0003] The laser confocal biochip analyzer currently in use uses fully mechanical two-dimensional scanning, dual light sources, and dual photodetectors to achieve dual-wavelength fluorescence detection. It includes dual light sources and dual photodetectors that are staggered at a certain angle. The laser beam emitted by the light source reaches a certain point on the biochip through lenses, beam splitters and prisms, and excites the ...

AI Technical Summary

Problems solved by technology

This biochip analyzer has the following disadvantages: 1) its scanning speed is limited by the operating frequency (up to about 20 Hz) of the linear drive, the analysis time of the biochip is long, and the vibration of the linear drive is large, the scanning inertia is large, and the reciprocating The stroke is long, resulting in a long stroke of the corresponding prism, which makes the focus spot of the laser beam change greatly, which affects the resolution of the instrument; 2) The numerical aperture NA of the condenser is limited by the mechanical structure, and the value is not very large, which affects the analysis of the instrument Sensitivity; 3) It uses dual light sources and dual detectors to realize simultaneous scanning and imaging of dual-wavelength fluorescence, which makes the structure of the entire instrument complex, and there is crosstalk in the scanning image, which affects the performance of the instrument

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