Cell culture device

Abstract

A cell culturing device with minimal death rate and maximal output of cells is disclosed. Its basic growth material has high adhesion of cells and large area. It can play the roles of oxygen-enriching unit, filter and static stirrer for periodically providing nutrients and oxygen to cells, resulting in higher output of cells.

Description

technical field [0001] The present invention is a cell culture device. When cultivating microorganisms, cells and / or tissues, the biggest obstacle is that the equipment and / or devices used are bulky, expensive, unable to be thrown away after use, difficult to sterilize and maintain a sterile environment , thus increasing the chance of contamination; and when culturing microorganisms, cells and / or tissues, it is easy to cause damage to microorganisms and cells and / or even cause cell death. The present invention provides a technique that can basically be used for culturing any microorganisms and cells in vitro and collecting any by-products produced therefrom. More specifically, the present invention protects and discloses a novel device and / or device and method that can effectively increase and collect by-products produced by microorganisms and cells, such as proteins. Background technique [0002] As we all know, large-scale cell culture procedures have been widely develope...

AI Technical Summary

Problems solved by technology

[0016] 1. In particular, the rate of oxygen diffusion through the flat disk-shaped permeable membrane becomes the main limiting factor for the size of the culture chamber

[0017] 2. Another disadvantage is that the flat disc-shaped permeable membrane is designed to be stretchable to have a stirring function, but this may cause cell death

Because Liau's device is complex and part of it is the equipment outside the culture room, such as external pipelines, storage tanks and pumps, it is very likely to introduce external pollution sources into the interior. In addition, because the device is huge, sterilization is not easy to carry out, and labor-intensive

[0023] 2. Another disadvantage of Liau's invention is that when the culture fluid flows through the system, the fluid shear stress caused may disturb and move the cells growing on the substrate plate, thereby reducing the survival rate of the cells

[0024] 3. In addition, the design of the vertical substrate plate is not conducive to cell attachment, because some cells that cannot be attached immediately will fall and accumulate at the bottom of the plate, and most of these cells will eventually die, thus reducing the survival rate of cells and protein production, and the system has to be restarted over and over again, which is rather inefficient and not ideal

[0025] 4. Furthermore, due to the complexity of this system, collecting any secreted proteins or cellular products is cumbersome and time-consuming

Finally, when the growth fluid is lower than the substrate plate on which the cells are grown, the cells will be exposed to air, i.e., directly in contact with a gaseous environment, thus resulting in cell death

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