Process for preparing high active powder composite green storage inoculating agent and its carrier adsorption agent

A kind of inoculant and powder technology, which is applied in the field of preparation of highly active powder compound silage inoculant and its carrier adsorption, can solve the problems of easy corruption and large loss of nutrients, and achieve the effects of promoting fermentation, improving quality, and expanding feed resources

Inactive Publication Date: 2006-06-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional silage technology loses a lot of nutrients during the silage process, and it is easy to rot after opening the cellar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of high active powder compound silage inoculant

[0029] Streptococcus lactis CVCC590 was used as the initial fermentation strain, and Lactobacillus plantarum AS1.557 was used as the subsequent fermentation strain.

[0030] After seed culture, the seed fermentation liquid is inoculated in the industrial production fermenter, and its culture medium is composed of: yeast 0.5-5%, 3-5% rice glucose syrup, mineral composition (content in 1 liter of culture medium) K 2 HPO 4 1-5g, 1-6g diammonium citrate, 2-8g sodium acetate, MgSO 4 ·7H 2 O 0.3-1g, MnSO 4 4H 2 O 0.1-1g.

[0031] Culture conditions

[0032] Seed culture: Simultaneous inoculation and asynchronous fermentation techniques are used to simultaneously insert Streptococcus lactis and Lactobacillus plantarum into a 10-liter seed tank for cultivation, using seed medium, the inoculum amount of each strain is 4% inoculum amount, and cultivated at 40°C Cultivate in box for 18 hours;

[0033] Industri...

Embodiment 2

[0040] Preparation of high active powder compound silage inoculant

[0041]Streptococcus lactis CVCC590 was used as the initial fermentation strain, and Lactobacillus Lactobacillus AS1.9 was used as the subsequent fermentation strain.

[0042] After seed cultivation, the seed fermentation liquid is inoculated into industrialized production fermenters, and its culture medium is composed of: 0.5-5% yeast, 3-8% corn glucose syrup, the remaining mass percentage is water, and the mineral composition ( content in 1 liter medium) K 2 HPO 4 1-5g, 1-6g diammonium citrate, 2-8g sodium acetate, MgSO 4 ·7H 2 O 0.3-1g, MnSO 4 4H 2 O 0.1-1g.

[0043] Culture conditions

[0044] Seed culture: adopt simultaneous inoculation and asynchronous fermentation technology, put Streptococcus lactis and Lactobacillus into 10 liter seed tanks for cultivation at the same time, use seed medium, the inoculation amount of each strain is 8% inoculum amount, 35 ℃ incubator Incubate for 18 hours.

[...

Embodiment 3

[0052] Streptococcus lactis and Lactobacillus were inoculated into the seed medium with the above-mentioned culture medium in this specification, and seeds were prepared in a 10-liter fermenter, and then inserted into a 150-liter fermenter for fermentation. The fermentation broth is subjected to continuous high-speed centrifugation to obtain the bacteria sludge, and then the protective agent, cellulase and carrier adsorbent are added according to the method in this manual, and the product is prepared into a powder passing through a 18-25 mesh sieve. Pack and seal with aluminum foil and store at 0-4°C.

[0053] The silage inoculant produced by this method is directly and evenly sprinkled in the silage corn stalks at an addition rate of 0.5 kg / ton.

[0054] project

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Abstract

The present invention belongs to the field of silage fermenting promoter technology. The present invention includes simultaneous inoculation of and asynchronous with of lactostreptococcus as initial fermenting bacteria and lactobacillus as subsequent fermenting bacteria, normal temperature carrier adsorption post-processing technology, optimizing the recipes of seed culture medium activating factor, industrial fermenting culture medium and protecting agent, and re-compounding fermented lactic acid bacteria and cellulase. The present invention has the features of mild production condition, low cost, high product activity, etc. The product may fit different silage materials and different silage conditions and can promote the fermentation of lactic acid bacteria and the homotypic fermentation, control negative fermentation, reduce loss of nutrients during fermentation and make full use of resource.

Description

technical field [0001] The invention relates to a high-activity powder compound silage inoculant and a preparation method for carrier adsorption thereof, belonging to the technical field of silage feed additives in the feed industry, and more specifically classified as silage fermentation accelerators. technical background [0002] The situation of 400 kilograms of grain per capita per year in my country will not change for a long period of time, that is to say, my country cannot produce more grain as feed grain. It is estimated that by 2000, the annual shortage of feed grains will reach 40 million tons. It has become a basic national policy of our country to use crop straw, forage, aquatic feed and other raw materials to prepare silage, develop feed resources, improve feed utilization, develop food-saving herbivorous livestock faster and faster, and increase meat production. [0003] my country is a large agricultural country, which produces more than 500 million tons of v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/25C12R1/46A23K3/03A23K30/18
Inventor 乐国伟李永富施用晖朱建津沈国强
Owner JIANGNAN UNIV
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