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Molecular blotting of organophosphorus parasiticide, method for preparation and application

A technology of molecular imprinting and organophosphorus, applied in botany equipment and methods, applications, biocides, etc., can solve the problems of high cost of determination, low recovery rate of pesticides, poor selectivity, etc., and achieve high selectivity, sensitivity, and recognition ability strong effect

Inactive Publication Date: 2006-07-12
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction methods of organophosphorus insecticides at home and abroad mostly use organic solvents to extract samples, and then go through column chromatography after liquid-liquid distribution. This method requires a lot of solvent and time, and the emulsification phenomenon is relatively serious; Large pesticides, such as methamidophos, dichlorvos, etc. are also easy to lose, and the determination accuracy is not high
In addition, many new technologies for pesticide extraction have been proposed recently, such as accelerated solvent extraction, solid-phase microextraction, supercritical fluid extraction, and purging distillation technology, etc., but these methods generally require special equipment, and the cost of determination is high, so it is difficult to promote application
Ordinary solid-phase extraction technology has been widely used in recent years as a pesticide pretreatment technology because it consumes less solvent and is convenient and quick, but the selectivity is relatively poor, and the recovery rate of some pesticides is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Preparation of Molecularly Imprinted Polymer MIP1 of Glyphosate

[0022] Weigh 0.507g template molecule glyphosate, 1.0320g reaction monomer methacrylic acid (MAA), 11.890g crosslinking agent ethylene glycol dimethacrylate (EGDMA), 0.0750g initiator azobisisobutyronitrile, 25ml of porogen chloroform, mixed evenly, put into a 40ml ampoule, blown with nitrogen gas for 5 minutes, sealed, and reacted in an oven at 70°C for 48 hours.

[0023] After polymerization, the polymer is taken out, ground, soaked with methanol, dried and sieved. First wash with 100ml of methanol, then ultrasonically clean with a mixed solution of 4×100ml methanol:acetic acid (volume ratio 8.5:1.5), and then ultrasonically clean with a mixed solution of 2×100ml methanol:triethylamine (volume ratio 99:1), Finally, wash with 5-8×100ml of methanol, concentrate each part of the eluent, and inject samples with high-performance liquid chromatography (UV), until no template molecules can be detec...

Embodiment 2

[0024] Example 2. Preparation of Molecularly Imprinted Polymer MIP2 of Chlorpyrifos

[0025] Weigh 0.3505g template molecule chlorpyrifos, 0.4305g reaction monomer methacrylic acid (MAA), 3.9600g crosslinking agent ethylene glycol dimethacrylate (EGDMA), 0.0100g initiator azobisisobutyronitrile, 10ml Pore ​​agent dichloromethane, mixed evenly, put into a 20ml ampule bottle, blown with nitrogen gas for 5 minutes, sealed, polymerized under a 365nm wavelength ultraviolet lamp, controlled the temperature at 8°C with a cold water bath, and reacted for 24 hours.

[0026]After polymerization, the polymer is taken out, ground, soaked in methanol, dried and sieved. First wash with 100ml of methanol, then ultrasonically clean with a mixed solution of 4×100ml methanol:acetic acid (volume ratio 8.5:1.5), and then ultrasonically clean with a mixed solution of 2×100ml methanol:triethylamine (volume ratio 99:1), Finally, wash with 5-8×100ml of methanol, concentrate each part of the eluate, ...

Embodiment 3

[0027] Example 3. Preparation of molecularly imprinted polymer MIP3 of dichlorvos

[0028] Weigh 0.2206g template molecule dichlorvos, 0.4305g reaction monomer methacrylic acid (MAA), 3.9640g crosslinking agent ethylene glycol dimethacrylate (EGDMA), 0.0420g initiator azobisisobutyronitrile, 10ml Pore ​​agent dichloromethane, mixed evenly, put into a 20ml ampule bottle, blown with nitrogen gas for 5 minutes, sealed, and reacted in an oven at 70°C for 48 hours.

[0029] After polymerization, the polymer is taken out, ground, soaked with methanol, dried and sieved. First wash with 100ml of methanol, then ultrasonically clean with a mixed solution of 4×100ml methanol:acetic acid (volume ratio 8.5:1.5), and then ultrasonically clean with a mixed solution of 2×100ml methanol:triethylamine (volume ratio 99:1), Finally, wash with 5-8×100ml of methanol, concentrate each part of the eluate, and inject into high-performance liquid chromatography ultraviolet detection until no template ...

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PUM

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Abstract

This invention discloses an organophosphorus insecticide molecular engram polymer and its process and application, wherein the process comprises the following steps: a, mixing one or more kinds of polymerization reaction monomers, cross agents, hole making agent, initiating agent and organophosphorus insecticide and then doing thermal initiating polymerization reaction or optical initiating polymerization reaction to get the polymer with organophosphorus; b, washing the polymer to get the molecular engram polymer without organophosphorus insecticide.

Description

technical field [0001] The invention relates to a molecularly imprinted polymer for organophosphorus insecticides and its preparation method and application, more specifically, to a molecularly imprinted polymer with specific recognition performance for organophosphorus insecticides and its The preparation method and the application of the molecularly imprinted polymer in separation, purification and determination of organophosphorus pesticides in biological or environmental samples. Background technique [0002] Organophosphorus pesticides are currently the most widely used and produced pesticides, and their residues are the most serious in agricultural products. The environmental pollution and food safety problems have also attracted people's attention. Organophosphorus pesticides (organophosphates) form phosphorylated cholinesterase with cholinesterase in the body, and the activity of cholinesterase is inhibited, making it unable to decompose acetylcholine, resulting in e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F20/00C08F26/00C08F2/44C08K5/49A01N57/00
Inventor 潘灿平李文明江树人
Owner CHINA AGRI UNIV
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