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Magnetic nano particle nucleic acid separator, and its preparing method and use

A magnetic nanoparticle, particle nucleic acid technology, applied in chemical instruments and methods, methods for granulating raw materials, preparation of sugar derivatives, etc., can solve the problems of microsphere agglomeration, affecting product performance, easy to fall off, etc.

Inactive Publication Date: 2006-11-15
SHANGHAI NORMAL UNIVERSITY
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Problems solved by technology

However, the structure of polymer microspheres is usually relatively loose, and the magnetic particles contained in it are easy to break away from the microspheres and agglomerate during long-term storage, thereby affecting the performance of the product; using adsorption or affinity on magnetic nanoparticles When fixing the active substance, it is easy to fall off

Method used

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  • Magnetic nano particle nucleic acid separator, and its preparing method and use
  • Magnetic nano particle nucleic acid separator, and its preparing method and use

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preparation example Construction

[0028] The method for preparing magnetic nanoparticle nucleic acid separator of the present invention comprises the following steps:

[0029] (1) Preparation of magnetic nanoparticles

[0030] Separately prepare FeSO with double distilled water 4 ·7H 2 O and FeCl 3 ·6H 2 O mixed solution and NaOH solution. Fe in a mixed solution of iron salts 2+ The ion concentration is 0.1~0.2mol / l, Fe 3+ The concentration of ions is 0.1-0.3 mol / l, and the concentration of NaOH solution is 2-3 mol / l. Under vigorous stirring, the NaOH solution whose volume is half of the volume of the mixed salt solution was slowly added dropwise into the mixed salt solution. Aging the obtained solid precipitate at 40°C to 60°C for 12 hours, washing the precipitate several times with twice distilled water, filtering and drying at 40°C to 80°C for 24 hours, grinding it in an agate mortar That is the product.

[0031] (2) Silica in γ-Fe 2 o 3 surface modification

[0032] Mix TritonX-100, n-hexanol a...

Embodiment 1

[0046] Preparation method of inner core layer of magnetic nanoparticles

[0047] The core layer of magnetic particles was prepared by improved chemical co-precipitation, the specific method is as follows: prepare FeSO 4 ·7H 2 O and FeCl 3 ·6H 2 O mixed solution and NaOH solution. In a mixed solution of iron salts, Fe 2+ The ion concentration is 0.1~0.2mol / l, Fe 3+ The concentration of ions is 0.1-0.3 mol / l, and the concentration of NaOH solution is 2-3 mol / l. Under vigorous stirring, the NaOH solution whose volume is half of the volume of the mixed salt solution was slowly added dropwise into the mixed salt solution. Aging the obtained solid precipitate at 40°C to 60°C for 12 hours, washing the precipitate several times with double distilled water, filtering and drying at 40°C to 80°C for 24 hours, and grinding it in an agate mortar Get the product.

Embodiment 2

[0049] Preparation method of core-shell nucleic acid magnetic nanoparticles

[0050] Mix TritonX-100, n-hexanol and cyclohexane uniformly at a ratio of 1:2:5 to form a transparent and stable microemulsion system. Put the microemulsion system above into ultrasonic treatment for 30-60 minutes, then add 0.5g of γ-Fe 2 o 3 After 6 minutes of ultrasonic treatment, the supernatant was taken out and poured into a three-necked flask, and stirred for 30 minutes to make it uniform. Take 1ml of a certain concentration of concentrated ammonia water and dilute it with 2ml of double distilled water, slowly add it into the microemulsion that is constantly stirring after 30 minutes, and keep stirring for 30 minutes to make the ammonia water evenly dispersed in the microemulsion. After 1 hour, 1-3 ml of tetraethyl orthosilicate was added dropwise to the microemulsion, while stirring continuously for 10 hours, and the temperature of the system was kept between 15-30°C. Add acetone to the sys...

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Abstract

The invention relates to a magnetic nanoparticle nucleic acid separator, which has the following three-layer structure: (1) an inner core layer composed of core-shell type magnetic nanoparticles; (2) 3-mercaptopropyltrimethoxy-coated core layer silane-based intermediate layer; (3) single-stranded DNA outer shell layer covering the intermediate layer. The present invention also relates to a method for preparing the magnetic nanoparticle nucleic acid separator. The magnetic nanoparticle nucleic acid separator of the present invention can simply and effectively separate the target DNA or RNA.

Description

technical field [0001] The invention relates to a nano biological separator, more specifically, the invention relates to a magnetic nano particle nucleic acid separator. The invention also relates to the preparation method and application of the separator. Background technique [0002] As we all know, the field of nanotechnology is the most popular field of science and technology research today. The emerging science and technology formed by applying nanotechnology to the field of biological sciences—nanobiotechnology is the use of nanotechnology to study and solve major problems in the field of life sciences. Science, it is becoming one of the current important frontier scientific research fields. [0003] Nowadays, the application prospect of magnetic nanoparticles in the field of biotechnology has attracted increasing attention. Magnetic nanoparticles are often used as separation media for magnetic field separation of biological samples. The sepa...

Claims

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Application Information

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IPC IPC(8): C07H21/00C07H1/06B01J2/00
Inventor 沈鹤柏汪友宝姜继森杨仲南
Owner SHANGHAI NORMAL UNIVERSITY
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