Flush applied organic bio-fertilizer
A flush fertilization and organic technology, applied in the field of bio-organic compound flush fertilization, can solve the problems of no bio-organic flush fertilization, influence the development of green agriculture, and fertilizer fertilization restrictions, and achieve the effects of long fertilizer efficiency period, maintaining a virtuous cycle, and simple use.
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[0028] 1. Production of bacterial agents:
[0029] (1) First, activate the original strains of Bacillus silicate, transfer the original strains to a nitrogen-free medium, and cultivate them at a temperature of 26-30°C for 48 hours. Bacteria contamination, as shake flask seeds.
[0030] (2) Inoculate the activated strains in the shake flask culture medium, shake at 26-30°C (reciprocating shaker 110 times / min), cultivate for 24 hours, check for no miscellaneous bacteria under the microscope, and use it as a fermenter kind. Capacity: 300-1000ml triangular flask, seed quantity: one slant strain inoculates three bottles.
[0031](3) Submerged fermentation: Carry out first-level seed fermentation, the seeds cultivated by the above-mentioned shake flasks are inserted into 50 liter seed tanks according to the inoculum size of 5%, and fermented for 24 hours at 26-30° C. Bacterial contamination is transferred to secondary 500-liter seed tanks. After 24 hours of fermentation, there is...
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