Nucleic acid test method for oligonucleotide microarray by fluorescence resonance energy transferring

Abstract

The nucleic acid detection method is characterized by that the nucleic acid sample to be detected and oligonucleotide microarray are hybridized and eluted to make the nucteic acid capable of completely pairing with oligonucleotide probe combine on the gene chip, and the sequence characteristics of oligonucleotide fragment on the oligonucleotide microarray are that the sequence of 5'0terminal and 3'-sequence of target area of nucleic acid sample to be detected are paired, sequence of 3'-terminal and 5'-sequence of target area are paired and middle sequence is oligomeric thymonucleic acid. The terminals are modified by different fluorescence groups respectively, and its middle is modified by using biotin. According to the wavelength of excitation light of fluorescence donor group and wavelength of emtting light of fluorescence receptor group it can detect the fluorescence signal and its position of microarray so as to can define the structural characteristics of said nucleic acid sample and its content.

Description

Technical field: [0001] The invention relates to a nucleic acid detection method based on fluorescence resonance energy transfer oligonucleotide microarray, which utilizes a novel gene chip for nucleic acid detection, and is used for clinical gene diagnosis of diseases, gene polymorphism analysis and species identification, etc., and belongs to biological detection technology field. Background technique: [0002] Gene chip technology is to immobilize gene probes on the surface of solid phase carrier in the form of microarray, and analyze the nucleic acid species and content of the sample solution to be tested according to the principle of nucleic acid base pairing. This technique has the advantages of miniaturization, parallel processing, and high throughput. Gene chips have been used in nucleic acid sequence analysis, gene expression profile analysis, single nucleotide polymorphism analysis, disease diagnosis, drug screening and other fields (Walker, Josef, Rigley, Kevin. ...

AI Technical Summary

Problems solved by technology

Since the nucleic acid to be tested must be labeled with a fluorescent group to be analyzed, on the one hand, the difficulty of the operation is increased. On the other hand, when the sample to be tested contains multiple nucleic acid samples to be analyzed, the difference between the labeled samples in a reaction solution makes the Inauthentic data, etc., limit the popularization and application of gene chips