Nucleic acid test method for oligonucleotide microarray by fluorescence resonance energy transferring

A fluorescence resonance energy and oligonucleotide technology, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of restricting the popularization and application of gene chips, untrue data, and increasing the difficulty of operation, etc. Low, simple operation, low interference effect

Inactive Publication Date: 2002-07-17
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

Since the nucleic acid to be tested must be labeled with a fluorescent group to be analyzed, on the one hand, the difficulty of the operation is increased. On the other hand, when the sample to be tested con

Method used

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Embodiment Construction

[0011] The technical solution of the present invention will be further described below through a specific embodiment.

[0012] In this example, the sex of the fetus is detected by fluorescence resonance energy transfer oligonucleotide microarray. The interaction between the protein and streptavidin immobilized the probe molecules on the glass slide. Fluorescein is excited with a wavelength of 490nm, and its emission wavelength is 518nm. If there is fluorescence energy resonance transfer, the emitted light of Fluorescein further excites Red640, which emits light at a wavelength of 640nm. During the detection process of the oligonucleotide microarray, the wavelength of the excitation light is 490nm, and the signal intensity of the microarray is detected at the wavelength of 640nm.

[0013] Fetal gender detection methods mainly include B-ultrasound and PCR detection. Two pairs of primers are used in the PCR method to detect fetal sex for amplification reaction. A pair of prime...

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Abstract

The nucleic acid detection method is characterized by that the nucleic acid sample to be detected and oligonucleotide microarray are hybridized and eluted to make the nucteic acid capable of completely pairing with oligonucleotide probe combine on the gene chip, and the sequence characteristics of oligonucleotide fragment on the oligonucleotide microarray are that the sequence of 5'0terminal and 3'-sequence of target area of nucleic acid sample to be detected are paired, sequence of 3'-terminal and 5'-sequence of target area are paired and middle sequence is oligomeric thymonucleic acid. The terminals are modified by different fluorescence groups respectively, and its middle is modified by using biotin. According to the wavelength of excitation light of fluorescence donor group and wavelength of emtting light of fluorescence receptor group it can detect the fluorescence signal and its position of microarray so as to can define the structural characteristics of said nucleic acid sample and its content.

Description

Technical field: [0001] The invention relates to a nucleic acid detection method based on fluorescence resonance energy transfer oligonucleotide microarray, which utilizes a novel gene chip for nucleic acid detection, and is used for clinical gene diagnosis of diseases, gene polymorphism analysis and species identification, etc., and belongs to biological detection technology field. Background technique: [0002] Gene chip technology is to immobilize gene probes on the surface of solid phase carrier in the form of microarray, and analyze the nucleic acid species and content of the sample solution to be tested according to the principle of nucleic acid base pairing. This technique has the advantages of miniaturization, parallel processing, and high throughput. Gene chips have been used in nucleic acid sequence analysis, gene expression profile analysis, single nucleotide polymorphism analysis, disease diagnosis, drug screening and other fields (Walker, Josef, Rigley, Kevin. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 刘建华刘喜朋陈洁林志新
Owner SHANGHAI JIAO TONG UNIV
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