Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tripterygium wilfordii regenerated plant and its preparation process

A technique for regeneration of plants and preparation process, applied in the biological field, can solve problems such as instability of active ingredients in culture, difficulty in large-scale culture, low yield of callus or suspension cell culture, etc.

Inactive Publication Date: 2002-09-04
上海延农生物工程有限公司 +2
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. Callus or suspension cells are undifferentiated cultures, while wild Tripterygium wilfordii uses plants as medicinal materials, which are differentiated products. There are essential differences between the two
[0006] 2. The culture yield of callus or suspension cells is low, and it is difficult to cultivate on a large scale for industrialization
[0007] 3. Callus or suspension cells are prone to genetic variation in long-term subculture, resulting in unstable active ingredients of the culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tripterygium wilfordii regenerated plant and its preparation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042]Example 1 Clonal Variation and Mutagenesis of Tripterygium wilfordii Cells or Callus

[0043] Take the young tissues of wild tripterygium wilfordii or seedlings as explants, sterilize with 75% ethanol for 30 seconds, sterilize with fenfenzolin or bleach for 15 minutes, wash with sterile water, cut into thin slices for induction culture, induce culture The base is 2 / 3MS, 2,4-dichlorophenoxyacetic acid (2,4-D) 1.0mg / L, naphthaleneacetic acid (NAA) 0.1mg / L, kinetin (KT) 0.1mg / L, 5% sucrose , 0.6% agar, cultured for 35 days to induce callus formation. Then carry out subculture of 2 to 3 generations, the subculture medium is: 2 / 3N6, hydrolyzed casein (CH) 500mg / L, 2,4-dichlorophenoxyacetic acid (2,4-D) 0.5mg / L L, indole butyric acid (IBA) 0.5 mg / L, kinetin (KT) 0.1 mg / L, 4% sucrose, 0.6% agar, each generation was cultured for 30 days to obtain a large number of callus. Put a large number of calli into liquid medium (2 / 3N 6 , 500mg / L hydrolyzed casein (CH), 2,4-dichlorophen...

Embodiment 2

[0046] Example 2 Plant Regeneration of Tripterygium wilfordii Suspension Culture Cells or Cell Clusters

[0047] The bright yellow suspension culture cells or cell clusters that have been subcultured or repaired are subjected to differentiation culture. The differentiation medium is 1 / 2MS, indole acetic acid (IAA) 0.1mg / L, kinetin (KT) 0.5mg / L, 6-benzylaminopurine (6-BA) 1.5mg / L, 3% sucrose, 0.6% agar, culture temperature is 22~25 ℃, and daily light is 12 hours, after 30 days, select thick normal seedling from regenerated seedling.

Embodiment 3

[0048] Example 3 Screening of Tripterygium wilfordii strains with high reproduction coefficient and active ingredient content

[0049] Carry out proliferation culture to the regenerated plants, the proliferation medium is 2 / 3MS, hydrolyzed casein (CH) 500mg / L, kinetin (KT) 0.5mg / L, 6-benzylaminopurine (6-BA) 0.5mg / L , naphthalene acetic acid (NAA) 0.1mg / L, 3% sucrose, 0.6% agar, select stout, fast-growing strains, get its part plants and carry out the mensuration of total diterpene lactone content (Lin Qishou etc., " Chinese herbal medicine component chemistry " 1977, page 390, Beijing, Science Publishing House), with the Tripterygium wilfordii extract sheet (batch number 20000505) of Leishi Pharmaceutical Shanghai No.

[0050] Select the high regenerated plant of total diterpene lactone content to carry out the mensuration of triptolide content again (Ye Xiaochuan, "Journal of Pharmaceutical Analysis", 1997, the 5th phase of 17 volumes: 319 pages), the standard sample of trip...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The preparation method of regenerative plant of thunder god vine mainly utilizes the techniques of clonal variation, mutation, chromosome doubling, regeneration and hormoneless propagation, etc. Saidinvention not only can make mass propagation, and the propagation coefficient of every generation can be up to 5-6 times, and the triptolide alcohol content is 0.009-0.015%, and its total diterpene lactone content also is higher than that of commercial thunder god vine extract, and they can be retained stably.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a regenerated plant of Tripterygium wilfordii and its preparation process. Background technique [0002] Tripterygium wilfordii Hook f. is a plant of the genus Tripterygium wilfordii Hook in the family Euonymus. Pharmacological experiments show that Tripterygium wilfordii has obvious anti-tumor, anti-inflammatory, immunosuppressive and anti-fertility effects. The current research results show that tripterygium wilfordii diterpene lactones are its main active ingredients. Tripterygium wilfordii has complex chemical components and extensive pharmacological effects, and has important clinical application value. [0003] However, because Tripterygium wilfordii is a perennial woody vine with slow growth, it has been widely used clinically, resulting in serious shortage of wild resources. Therefore, the artificial cultivation of Tripterygium wilfordii plays an ine...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 黄剑华陆瑞菊孙月芳王亦菲周润梅俞庆全
Owner 上海延农生物工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com