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Use of tanshin polyphenolic acid B in preparing tumor curing medicine

A tumor drug, salvianolic acid technology, applied in the field of medicine, can solve the problems of limited curative effect, patient side effects, high recurrence rate, etc.

Inactive Publication Date: 2003-04-09
上海天甲生物医药有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the recurrence rate of malignant tumors after surgery has remained high. Chemotherapy and radiotherapy not only bring devastating side effects to patients, but also often have limited curative effects due to drug resistance and low sensitivity.

Method used

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  • Use of tanshin polyphenolic acid B in preparing tumor curing medicine
  • Use of tanshin polyphenolic acid B in preparing tumor curing medicine
  • Use of tanshin polyphenolic acid B in preparing tumor curing medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Extraction method of salvia miltiorrhiza

[0017] Salvia miltionrrhiza Bge. is crushed into 100-mesh powder after removing impurities such as silt, heated and extracted three times with water, combined with water extracts, concentrated under reduced pressure, and added alcohol (methanol, ethanol) to the concentrated solution to 70-80% alcohol content , let cool overnight; remove the precipitate with a centrifuge, remove the alcohol from the solution, pass through a macroporous resin (type Diaion HP20 or 400-1), elute with water and ethanol, collect the phenolic acidic part, concentrate to a small volume, and pass through the gel Column (MCI gel, TSK HF40w, SephadexLH-20, Silica gel ODSG3, etc.), eluted with water-ethanol, and collected the eluate containing part B of salvianolic acid. The eluate was evaporated in vacuum to remove ethanol, and freeze-dried to obtain salvianolic acid B dry powder.

Embodiment 2

[0019] Dose-effect relationship graph of salvianolic acid B inhibiting tumor cell growth

[0020] Human liver cancer cell lines SK-Hepl, Huh7, SMMU-7721, mouse liver cancer cell line H22, and human cervical cancer cell line Hela were selected for experiments (except Hela cell lines were purchased from the Cell Bank of Shanghai Institute of Cell Biology, Chinese Academy of Sciences, the above All cell lines were donated by Reid Institute for Liver Diseases). The above cells were cultured with RPMI-1640 (purchased from Sigma) medium containing 10% calf serum, and the monolayer cells were digested with 0.25% trypsin (purchased from Sigma) to prepare cell suspension, and the cell concentration was adjusted to 1 ×10 5 / ml. Take a 96-well culture plate, add 100ul of cell suspension to each well, and culture overnight to make the cells adhere to the wall. Aspirate the culture solution, add the culture solution containing salvianolic acid B, the final concentration of salvianolic a...

Embodiment 3

[0022] Therapeutic effect of salvianolic acid B on inoculated tumors in animals

[0023] Take 20 C57 mice (both male and female, body weight 18-22g, purchased from Shanghai Experimental Animal Center), and each mouse was subcutaneously inoculated with a concentration of 1×10 7 / ml of Hep1-6 liver cancer cells; 16 days later, tumors with a diameter of 1-2cm grew in the mice. At this time, the mice were divided into two groups, namely a control group and a treatment group, with 10 mice in each group. From the 17th day, mice in the treatment group were injected intraperitoneally with salvianolic acid B solution, once a day, and the dose was 100 mg / kg (the concentration of salvianolic acid B solution was 5 mg / ml, prepared with normal saline); An equal volume of normal saline was injected once as a control. Such as figure 2 As shown, the results showed that the survival time of mice in the treatment group was prolonged.

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Abstract

The present invention provides a new application of tanshin polyphenolic acid B in preparing medicine for treating tumor. Extraneous research shows that tanshin polyphenolic acid B has the function of killing tumor cell and animal test shows its antineoplastic effect. In the present invention, the cation in the salt of tanshin polyphenolic acid B may be Fe ion, Na ion, K ion, Ca ion or Mg ion, preferably Mg ion. The said medicine may be in any medicine form, oral liquid, injection, tablet or capsule.

Description

Technical field: [0001] The invention relates to the field of medicine, in particular to the application of salvianolic acid B, especially the application of salvianolic acid B in the preparation of drugs for treating tumors. Background technique: [0002] Surgery, chemotherapy and radiotherapy are the three traditional treatments for malignant tumors. However, the recurrence rate of malignant tumors after surgery has remained high. Chemotherapy and radiotherapy not only bring devastating side effects to patients, but also often have limited efficacy due to the emergence of drug resistance and low sensitivity. [0003] Salvia miltiorrhiza is a traditional Chinese medicine in my country, which has the effects of promoting blood circulation and removing blood stasis, dredging meridians and activating collaterals. Salvianolic acid B is one of the water-soluble active ingredients of Danshen, and its chemical structure is as follows: [0004] Among them, F is a metal cation. ...

Claims

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Application Information

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IPC IPC(8): A61K31/343A61P35/00
Inventor 谢天培顾心彬季红光
Owner 上海天甲生物医药有限公司
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