Use of tanshin polyphenolic acid B in preparing tumor curing medicine
A tumor drug, salvianolic acid technology, applied in the field of medicine, can solve the problems of limited curative effect, patient side effects, high recurrence rate, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0015] Example 1
[0016] Salvia miltiorrhiza extraction method
[0017] Salvia miltionrrhiza Bge. is pulverized into 100 mesh powder after removing impurities such as sediment and heating with water for three times. The water extracts are combined and concentrated under reduced pressure. The concentrated solution is added with alcohol (methanol, ethanol) to 70-80% alcohol content , Let cool overnight; remove the precipitate with a centrifuge, remove the alcohol from the solution, eluting the macroporous resin (model Diaion HP20 or 400-1) with water and ethanol, collect the acidic part of the phenol, concentrate to a small volume, and then pass through the gel Column (MCI gel, TSK HF40w, Sephadex LH-20, Silica gel ODSG3, etc.), eluted with water-ethanol, and collected the eluate containing salvianolic acid B. The eluate was vacuum rotary evaporated to remove ethanol, and freeze-dried to obtain salvianolic acid B dry powder.
Example Embodiment
[0018] Example 2
[0019] Diagram of the dose-effect relationship of salvianolic acid B inhibiting tumor cell growth
[0020] Select human liver cancer cell lines SK-Hepl, Huh7, SMMU-7721, mouse liver cancer cell line H22, human cervical cancer cell line Hela for experiments (except for the Hela cell line purchased from the cell bank of the Shanghai Institute of Cell Biology, Chinese Academy of Sciences, the above The cell lines are all donated by the Reid Institute of Liver Diseases). The above cells were cultured with RPMI-1640 (purchased from Sigma) medium containing 10% calf serum, and the monolayer cells were digested with 0.25% trypsin (purchased from Sigma) to prepare a cell suspension, and the cell concentration was adjusted to 1 ×10 5 / ml. Take a 96-well culture plate, add 100ul cell suspension to each well, and incubate overnight to make the cells adhere to the wall. Aspirate the culture medium and add the culture medium containing salvianolic acid B. The final concentra...
Example Embodiment
[0021] Example 3
[0022] Therapeutic effect of salvianolic acid B on animal inoculated tumors
[0023] Take 20 C57 mice (both male and female, weighing 18-22g, purchased from Shanghai Experimental Animal Center), and each mouse was inoculated subcutaneously at a concentration of 1×10 7 / ml Hep1-6 liver cancer cells; 16 days later, tumors with a diameter of 1-2 cm grow in the mice. At this time, the mice were divided into two groups, namely the control group and the treatment group, with 10 mice in each group. From the 17th day, the mice in the treatment group were injected intraperitoneally with salvianolic acid B solution once a day at a dose of 100 mg / kg (the concentration of salvianolic acid B solution was 5 mg / ml, prepared with normal saline); mice in the control group were intraperitoneally injected daily An equal volume of normal saline was injected once as a control. Such as figure 2 As shown, the results indicate that the survival time of the mice in the treatment group ...
PUM
Property | Measurement | Unit |
---|---|---|
Diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap