Solidoid ferment method utilizing low value old rice as main material for fungal spore powder of hyphomycetales

A technology for fungal spores and main raw materials, applied in the field of solid-phase fermentation, can solve the problems of low spore content in the original powder, increase the production cost of spore powder, etc., and achieve the effects of eliminating bacterial pollution, convenient management and simplified process flow

Inactive Publication Date: 2003-05-07
ZHEJIANG UNIV
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Problems solved by technology

[0006] 3) Chinese invention patent CN1149393A (disclosure date: 97-05-14) discloses a method of using brewery waste to produce Paecilomyces lilacinus mycelium preparation to prevent and control crop nematodes, and does not involve the use of solid-phase fermentation and aerial components of rice Spore
[0025] 23) European patent EP0406103 (publication date: 91-01-02) discloses a method for preparing fungal insecticide powder by mixing Beauveria bassiana and kaolin, and does not involve the technology of solid-phase fermentation with rice
Because the spore content of the original powder is too low, further purification is required to reach 100 billion / g to meet the preparation requirements of high-standard spore preparations, thus greatly increasing the production cost of spore powder

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0049] Adopt the method of the present invention to produce the high-purity aerial conidia powder of Beauveria bassiana coccidioides SG8702 bacterial strain, use 6 kilograms of rice, through sterilizing ripening, insert the mycelia liquid of liquid phase fermentation 48 hours and add additive in proportion, pack After 6 days of solid fermentation at 25°C and drying at 35°C for 1 day to pass through a 120-mesh sieve, then vacuum-dry at room temperature for 12 hours to obtain 189 grams of spore powder (flour production rate 3.15%), with a water content of 5.25 %, the spore content is 148 billion / gram, and the live spore rate is 98.7%. Example 2

Embodiment 2

[0050] Adopt the inventive method to produce the high-purity aerial conidia powder of Paecilomyces fumigatus Pfr116 bacterial strain, use 7 kilograms of rice, through sterilizing ripening, insert the mycelia liquid of liquid-phase fermentation 48 hours and add additive in proportion, After packing, pass 120 mesh sieve through 8 days of solid fermentation at 25°C and dry at 35°C for 1 day, then vacuum-dry at room temperature for 18 hours to obtain 187.6 grams of spore powder (flour production rate 2.68%), with a water content of 3.16%, the spore content is 135.2 billion / g, and the live spore rate is 91.2%.

Embodiment 3

[0052] Adopt the inventive method to produce the high-purity aerial conidia powder of Metarhizium anisopliae ZD-15 bacterial strain, use 0.85 kilograms of rice, through sterilizing ripening, insert the mycelia liquid of liquid phase fermentation 48 hours and add additive in proportion, After packing, ferment for 9 days at 25°C and dry at 35°C for 1 day to pass through a 120-mesh sieve, then vacuum-dry at room temperature for 24 hours to obtain 27.65 grams of spore powder (yield 3.25%), with a water content of 4.16 %, the spore content is 121.5 billion / g, and the live spore rate is 92.3%.

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Abstract

A solid-phase fermenting method for using the low-value old rice as the main raw material of hyphomycetous spore powder includes such steps as immersing the low-value old rice, steam sterilizing, half-cooking, cooling, proportionally mixing with liquid-phase fermented hypha liquid (or the spore suspension and additive, culturing, baking and sieving. Its advantages are simple process, no bacterialpollution, short period, high purity of spore powder, and low cost.

Description

technical field [0001] The invention relates to a solid-phase fermentation method in which low-value aged rice is used as the main raw material of hyphosporium fungus spore powder. Background technique [0002] Research and development of microbial agents with effective infectants as active ingredients to control crop pests, insecticidal fungi are important resources available, because only fungi can infect and kill pests through body wall contact under natural conditions, not only for piercing and sucking effective against chewing mouthparts and also against chewing mouthparts. At present, there are dozens of Entomopathogenic hyphomycetes known, but the strains that are mainly researched and developed at home and abroad are mainly concentrated in a few species such as Beauveria bassiana, Metarhizium anisopliae, and Paecilomyces. According to existing research reports, the conidia (effective infectants) of insecticidal fungi can attach to the surf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04C12M1/16C12N1/14C12N3/00
Inventor 冯明光张丽靖应盛华
Owner ZHEJIANG UNIV
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