L-arginine producing strain and its mutation method and usage in producing L-arginine

An arginine and high arginine technology, applied in bacteria, fermentation and other directions, can solve the problems of difficult process amplification, single structural analog, reverse mutation, etc., and achieve improved genetic stability, good genetic stability, and yield traits. stable effect

Inactive Publication Date: 2003-09-10
JIANGNAN UNIV
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Problems solved by technology

The mutagenesis methods and structural analogues used in the above method are often relatively simple, which may easily cause poor genetic stability of mutant strains with high yield of L-arginine, and produce back mutations in large-scale production, thereby causing the decline of L-arginine yield traits
Fermentative production of L-arginine, there is also the use of Escherichia coli or Corynebacterium glutamicum through genetic modification to introduce the gene of the key enzyme or in

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  • L-arginine producing strain and its mutation method and usage in producing L-arginine
  • L-arginine producing strain and its mutation method and usage in producing L-arginine

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Embodiment 1

[0032] Preparation of mutants resistant to sulfaguanidine, D-arginine, homoarginine and methylcysteine ​​and fermentative production of L-arginine.

[0033] The starting strain SYA5 was subjected to ultraviolet mutagenesis and diethyl sulfate mutagenesis to obtain the SD100-28 strain, and the nitrosoguanidine mutagenesis was continued, and the SD100-28 strain was inserted into the seed medium and cultivated until the middle and late logarithmic growth stages. 50% of the inoculum was transferred to fresh seed medium and cultured for 5 hours to achieve synchronous culture of the cells. Aseptically centrifuge the culture solution, collect the bacteria, wash with 0.1mol / l, pH6.0 phosphate buffer for 2 to 3 times, transfer to a Erlenmeyer flask with glass beads to disperse, obtain a bacterial suspension, and control the cell concentration at 10 8A / ml or so. Weigh a small amount of nitrosoguanidine and dissolve it in a small amount of acetone, then add phosphate buffer to prepare ...

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Abstract

The present invention relates to the fermentation process of producing L-arginine. By using Corynebacterium crenatum SYA5 screened and preserved by the present lab as parent and through conventional physical and chemical mutation process and multiple structural analog resistance screening, mutant strain SDNN403 is obtained. Through culturing of the mutant strain under optimized condition, L-arginine is produced in the yield level of 30-35 g/L. The strain has high genetic stability, stable yield characteristic, high L-arginine yield level, less produced hetero acids and easy technological amplification, and is suitable four industrial production.

Description

technical field [0001] A bacterial strain producing L-arginine, its mutagenesis method and a method for producing L-arginine using the bacterial strain. The present invention relates to using glucose and ammonium sulfate as main raw materials and utilizing Corynebacterium crenatum to mutate A method for producing L-arginine by strain fermentation. Background technique [0002] L-arginine is a semi-essential amino acid for human body and animals. It has important biochemical and physiological significance, so it is widely used in food and pharmaceutical industries, especially in the field of pharmaceutical industry. Medicines for the treatment of vascular diseases and enhancing sperm motility in men have received attention. [0003] The preparation methods of L-arginine include extraction method (Chinese patent CN1161959) and fermentation method. The extraction method is time-consuming, has low yield, serious environmental pollution, and is not suitable for large-scale produ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/10
Inventor 陶文沂许正宏熊筱晶窦文芳史劲松
Owner JIANGNAN UNIV
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