Chinese yew sprout tissue culture medium and its sprout breeding method

A technology of tissue culture medium and yew, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of inability to form industrialized production, no artificial tissue culture, low germination rate and survival rate, etc. Achieve the effects of low cost, simple and practical components, and short incubation time

Inactive Publication Date: 2003-11-12
曹家骙 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the reproductive characteristics of Taxus chinensis tissue culture, general conventional methods are difficult to work, so there are no literature records on artificial tissue culture and efficient regeneration plant bre

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0012] Example 1 of the present invention: Take nutrient: "MS" medium 90%, growth promoter: gibberellin + ethanol 1.9%, stabilizer: gelatin 8%, trace elements: papain and griseofulvin 0.1% , Mixed to make a culture medium; then add the skewer of about 0.6cm in length from the twigs of the yew mother tree (with 1-2 shoots) that were born and semi-lignified in the same year, at an ambient temperature of 24±4℃, and a humidity of 80~ 85%, light intensity 1850LX, 11 hours of light per day, about 10 days can produce roots, form regenerated plants, and its batch survival rate is 83%.

Example Embodiment

[0013] Example 2: Take nutrient: "N8" medium 88.5%, production promoter: gibberellin + ethanol 2%, stabilizer: gelatin + agar 9%, trace elements: papain and griseofulvin 0.5%, After mixing, make a culture medium; add the tissue cytoplasm after mechanical crushing and pulping of 1-year-old or non-lignified yew twigs (buds) leaves, light for 14 hours a day (10 hours after 5 days), light intensity 1900LX (5 2000XL), the ambient temperature is 24±4℃, the humidity is 84~88%, a single seedling can be formed in 15~25 days, and it can be transplanted into the culture tube (2 seedlings) after 25 days, the batch survival rate is 30%~40 %.

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Abstract

The invention discloses a Chinese yew-seedling tissue culture medium and the breeding method, made up of nutritious agent, growth promoter, stabilizing agent and microelement. It mechanically crushes tender spike branches or tender branches (buds) and leaves of the parent-body tree, then adds their crushed histiocytic plasm into the culture medium for cultivating in groups. It has simple preparing process, low cost, little loss, short cultivating time, etc. The cultivating time of the spike branches 7-15 days, and that of the histiocytic plasm 10-25 days.

Description

Technical field: [0001] The invention relates to a culture medium and a breeding method for plants. technical background: [0002] Taxus is a rare tree species in the world with very few natural resources. Since it was discovered in the 1980s that it contains paclitaxel, a natural and highly effective anticancer drug, it has attracted widespread attention. However, due to the reproductive characteristics of yew tissue culture, general conventional methods are difficult to be effective, so there are no literature records on artificial tissue culture and efficient regeneration plant breeding. If the traditional seed method or cutting method is used, due to the limitation of seedling resources, coupled with low germination rate and survival rate, and slow growth period, industrialized production cannot be formed. Invention content: [0003] The purpose of the present invention is to provide a tissue culture medium and a high-efficiency cultivation method with simple componen...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 曹家骙朱诗庆
Owner 曹家骙
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