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Chinese yew sprout tissue culture medium and its sprout breeding method

A technology of tissue culture medium and yew, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of inability to form industrialized production, no artificial tissue culture, low germination rate and survival rate, etc. Achieve the effects of low cost, simple and practical components, and short incubation time

Inactive Publication Date: 2003-11-12
曹家骙 +1
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AI Technical Summary

Problems solved by technology

However, due to the reproductive characteristics of Taxus chinensis tissue culture, general conventional methods are difficult to work, so there are no literature records on artificial tissue culture and efficient regeneration plant breeding.
If the traditional seed method or cutting method is used, due to the limitation of seedling resources, coupled with the low germination rate and survival rate, the growth period is slow, and industrial production cannot be formed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1 of the present invention: Nutrient: "MS" medium 90%, growth promoter: gibberellin+ethanol 1.9%, stabilizer: gelatin 8%, trace element: papain and griseofulvin 0.1% , mixed to make a culture medium; then add the semi-lignified yew mother tree twigs (with 1 to 2 buds) about 0.6cm long, at an ambient temperature of 24±4°C and a humidity of 80~ 85%, the light intensity is 1850LX, and the light is 11 hours a day, and the root system can be produced in about 10 days to form regenerated plants, and the batch survival rate is 83%.

Embodiment 2

[0013] Embodiment 2: Get nutrient: "N8" medium 88.5%, production accelerator: gibberellin+ethanol 2%, stabilizer: gelatin+agar 9%, trace element: papain and griseofulvin 0.5%, Make culture medium after mixing; Add 1-year-old or non-lignified Taxus twigs (buds) leaves mechanically pulverized and pulped tissue cell pulp, light 14 hours a day (10 hours after 5 days), light intensity 1900LX (5 Tianhou 2000XL), ambient temperature 24±4°C, humidity 84-88%, a single larva can be formed in 15-25 days, and can be transplanted into the culture tube after 25 days (2 seedling cultivation), the batch survival rate reaches 30%-40 %.

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PUM

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Abstract

The invention discloses a Chinese yew-seedling tissue culture medium and the breeding method, made up of nutritious agent, growth promoter, stabilizing agent and microelement. It mechanically crushes tender spike branches or tender branches (buds) and leaves of the parent-body tree, then adds their crushed histiocytic plasm into the culture medium for cultivating in groups. It has simple preparing process, low cost, little loss, short cultivating time, etc. The cultivating time of the spike branches 7-15 days, and that of the histiocytic plasm 10-25 days.

Description

Technical field: [0001] The invention relates to a culture medium and a breeding method for plants. technical background: [0002] Taxus is a rare tree species in the world with very few natural resources. Since it was discovered in the 1980s that it contains paclitaxel, a natural and highly effective anticancer drug, it has attracted widespread attention. However, due to the reproductive characteristics of yew tissue culture, general conventional methods are difficult to be effective, so there are no literature records on artificial tissue culture and efficient regeneration plant breeding. If the traditional seed method or cutting method is used, due to the limitation of seedling resources, coupled with low germination rate and survival rate, and slow growth period, industrialized production cannot be formed. Invention content: [0003] The purpose of the present invention is to provide a tissue culture medium and a high-efficiency cultivation method with simple componen...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 曹家骙朱诗庆
Owner 曹家骙
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