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Method for preparation of optical biochemical sensor devices

A chemical sensor, sensor technology, applied in chemical instruments and methods, measuring devices, scientific instruments, etc., can solve problems such as difficulty in reproducing the size of deposited liquid spots, incompatibility, etc.

Inactive Publication Date: 2004-06-30
VIR AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fiber tube-printer is not suitable for the deposition of polymer and polymer precursor liquids with high viscosity, because the acceptance of the liquid is based on the absorption of the liquid into the fiber tube, and the problems mentioned above may arise
Another disadvantage is that it is very difficult to reproduce the dot size of the deposited liquid, e.g. some commercially available printers require a pre-printing step to obtain a constant dot size

Method used

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  • Method for preparation of optical biochemical sensor devices
  • Method for preparation of optical biochemical sensor devices
  • Method for preparation of optical biochemical sensor devices

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Improvement of Commercially Available "Pin-Ring" Array Printer

[0115] A commercially available "needle-and-ring" array printer (Affymetrix 417, formerly from Genetic Microsystem as GMS 417) was modified to accommodate deposition involving polymers or polymer precursors rather than biological or biochemical liquids, i.e. modified for use with components such as ethanol Continuous use of organic solvents to rinse the needle. Tubing is usually silicone, changed to more solvent resistant FEP (fluoroethylene-propylene) tubing. In a similar manner, the pump (AS Thomas) delivering the flushing liquid to the flushing station was removed and replaced by a "chemical resistant" type of the same type. The guard lock of the door was disabled so that the needle could be washed manually with tetrahydrofuran using a wash bottle. Flow restrictors are installed on the rinse solvent lines instead of (or in addition to) the clamps to enhance control of the solvent sprayed out of the ...

Embodiment 2

[0117] Prepare many tiny PVC dots on glass material

[0118] Dissolve 33 mg of poly(vinylamine) (PVC) (high molecular weight) and 66 mg of plasticizer bis(2-ethylhexyl) sebacate in 800 μL of cyclohexanone, and fill 35 μL of the resulting spotting liquid into 256 wells Well A1 of a polypropylene microtiter plate. Typical arrays of PVC dots can be easily deposited on substrates such as commercially available glass or gold-coated glass microscope slides ( figure 1 ). Other support surfaces can be placed in the container by using conventional metal liners. To remove PVC-DOS residue, the needle was rinsed with tetrahydrofuran. This can be done manually, or a suitable solvent can be used in the rinse tube and bath in a correspondingly modified instrument.

Embodiment 3

[0120] Fabrication of many tiny sodium-selective (bio)chemical sensor dots

[0121] 2.9 mg of 9-(diethylamino)-5-octadecanoylimino)-5H-benzo[a]phenoxazine, 4.6 mg of tetrakis[3,5-bis(trifluoromethyl) Sodium phenyl]borate, 10.0 mg of 4-tert-butylcalix[4]arene-tetraethyl tetraacetate, 139.2 mg of bis(2-ethylhexyl) sebacate, and 69.1 mg of poly(vinyl chloride) (high molecular weight) was dissolved in 2.0 ml cyclohexanone. 35 μL of the resulting spotting liquid was filled into well A1 of a 256-well polypropylene microtiter plate. Plasticized PVC-based sodium-selective (bio)chemical sensor dots were fabricated on gold-coated microscope slides using a GMS417 array printer with 125 μm needles. The functionality of the sensor dots, i.e. responsive to the target ion sodium in buffer solution, can be demonstrated using fiber optic absorptivity spectroscopy or surface plasmon resonance spectroscopy, respectively. The latter detects refractive index changes in thin films, which are r...

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Abstract

The present invention relates to a method for the preparation of a miniaturized optical chemical or biochemical sensor device (e.g. bulk optode, etc. for ion sensing), said device comprising a substrate material having a planar surface portion, said planar surface representing a transducer based on an optical phenomenon such as surface plasmon resonance based on evanescent waves, reflection or transmission; said planar surface portion having arranged thereon an multi-analyte array of (bio)chemical sensor dots located at spatially separated predetermined positions of the planar surface, said sensor dots including (i) a polymer matrix, and (ii) one or more (bio)chemical recognition moieties, the method comprising (a) providing a substrate material having a planar surface portion; (b) providing one or more spotting fluid(s); (c) depositing the one or more spotting fluid(s) onto the planar surface portion of the substrate material by means of a pin-printer deposition mechanism (arrayer) and allowing the spotting fluid(s) to consolidate.

Description

technical field [0001] The present invention relates to the preparation of photo(bio)chemical sensor devices for simultaneous monitoring of a large number of different compounds. Other possible uses are high-yield screening of combinatorial libraries, food quality monitoring, process control, gene expression monitoring, and detection of biological components, among others. More specifically, the present invention relates to methods for the preparation of photo(bio)chemical sensor devices comprising a plurality of polymeric (bio)chemical sensor spots. Background technique [0002] Trends in the field of chemical and biochemical sensors [R. Kellner, M. Otto, M. Wldmer, Analytical Chemistry: The Approved Text to the FECSCurriculum Analytical Chemistry, Wiley-VCH 1998, pp. 359-360 and pp. 375ff. ] is a new way to improve and develop methods for performing typical analyzes to meet the growing demand for high-capacity analysis such as environmental and clinical samples as well as...

Claims

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Application Information

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IPC IPC(8): G01N21/64B01J19/00C40B40/06C40B40/10C40B60/14G01N21/27G01N21/552G01N21/59G01N33/543G01N35/10G01N37/00
CPCB01J2219/00677B01J2219/0059C40B40/06G01N21/553B01J2219/00743B01J2219/00725B01J2219/00722B01J2219/00612G01N21/648B01J2219/00387B01J2219/00585B01J2219/00659B01J2219/00497B01J2219/00617B01J2219/00596B01J2219/00637B01J2219/0074B01J2219/00605B82Y30/00B01J2219/00626B01J19/0046C40B40/10B01J2219/00527C40B60/14B01J2219/00729B01L3/00
Inventor U·雷德尔A·F·斯坦格C·西斯特鲁普
Owner VIR AS
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