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Feed supplementing optimization method of saccharomyces pichia fermentation batch process

A technology of Pichia pastoris and optimization method, applied in the biological field, can solve problems such as not involving yield optimization, and achieve the effect of improving protein yield

Inactive Publication Date: 2004-12-22
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

Also found in the retrieval, Ren et al., Macrokinetic model formethylotrophic Pichia pastoris based on stoichiometric balance, Journal of Biotechnology, 106: 53-68, 2003 (Ren Haitao et al., based on the macrokinetic model of Pichia pastoris based on stoichiometric balance, Journal of Biotechnology , 106:53-68, 2003) established a macroscopic kinetic model of Pichia pastoris, but the article did not involve yield optimization

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  • Feed supplementing optimization method of saccharomyces pichia fermentation batch process
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Embodiment Construction

[0016] In conjunction with the content of the method of the present invention, the following examples are further provided:

[0017] Object: Pichia pastoris GS115 recombinant human serum albumin fermentation batch process. The fermentor volume is 10 (liters), and the glycerol batch fermentation period and the glycerin fed-batch fermentation period are carried out by the operation specification provided by Invitrogen Company, the initial fermented liquid volume of the methanol fed-batch fermentation period is 4 (liters), and the methanol induction period is 20( Hour).

[0018] Control objective: keep the cell specific growth rate constant at 0.006 (per hour) during the methanol fed-batch fermentation period.

[0019] Specific steps are as follows:

[0020] (1) In the methanol induction period-be positioned at the initial stage of methanol fed-batch fermentation-at first implement the linear feeding method, and its equation is:

[0021] F = ω 1 +ω 2 *(t-t f ) (t f ≤t≤t f...

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Abstract

An optimized method for supplementing raw material in the Pichia yeast fementing procedure features that in the fermenting phase adding methanol in flowing mode, a linear increment method is first used to supplementing methanol to make the specific growth rate of cell reach a predefined value, and a parabolic method is then used to make the specific growth rate of cell contant. For the recombinant human serum albumin expression system with Pichia yeast GS115, it can obviously increase the output rate of albumin.

Description

technical field [0001] The invention relates to an optimization method, in particular to a feeding optimization method for a batch process of recombinant human serum albumin produced by fermentation of Pichia pastoris, belonging to the field of biotechnology. Background technique [0002] Human serum albumin is a polypeptide chain composed of 585 amino acids with a molecular weight of 66.5kDa. The concentration of serum albumin in human plasma is as high as 42gl -1 Clinically, human serum albumin is mainly used as a plasma volume expander, rescue of patients with shock, burns and blood loss, and adjuvant therapy for cancer. At present, human serum albumin products are mainly separated and produced from human plasma or placenta, and the products are more likely to be contaminated by blood pathogenic viruses (such as hepatitis B virus and AIDS virus). In recent years, with the development of recombinant DNA technology, the production of human serum albumin by biotechnology h...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02
Inventor 袁景淇任海涛
Owner SHANGHAI JIAO TONG UNIV