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Beneficial bacteria preparation for poultry and its preparation method

A technology of probiotics and bacterial preparations, applied in applications, animal feed, animal feed, etc., can solve the problems of density and activity to be improved, achieve the effects of enhancing immunity and disease resistance, increasing conversion rate, and improving environmental quality

Inactive Publication Date: 2005-03-23
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its density and activity still need to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Carry out the preparation of bacterial preparation according to the following steps:

[0022] 1) Activate the slant surface of each bacterial strain stored at 4°C, and culture them in shake flasks (200ml-2L). Bacillus, yeast and actinomycetes are cultivated with soluble starch medium, and the specific formula is as follows: soluble starch 20g, KNO 3 1g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 .7H2O 0.5g, FeSO 4 0.01g, 15-20g agar, 1000ml distilled water, pH7.3, autoclave at 121°C for 20 minutes. Lactic acid bacteria are directly cultured with sterilized fresh milk. Bacillus and yeast, cultured at 30°C for 22 hours; actinomycetes and lactic acid bacteria, cultured at 30°C for 48 hours.

[0023] 2) Level-1 liquid expansion culture (Bacillus, yeast, actinomycetes and lactic acid bacteria are separately expanded culture, medium and culture conditions are the same as above) (20L tank), inoculum size 15%.

[0024] 3) Secondary liquids are respectively expanded for culture ...

Embodiment 2

[0029] Mix the bacteria preparations prepared above in the following proportions: 600 kg of bacillus, 200 kg of actinomycetes, 200 kg of lactic acid bacteria, and 10% inactivated yeast. save.

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PUM

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Abstract

A useful bacteria feed for fowls is prepared from bacillus, actinomycess, lactobacillus and deactivated yeast through proportional mixing, stage-one enlarge culture in liquid, stage-two enlarge culture in liquid immobilizing them on the surface of wheat bran, drying, and pulverizing.

Description

technical field [0001] The invention relates to a poultry probiotic preparation and a preparation method thereof. Background technique [0002] Probiotic preparations are poultry feed additives, which contain a variety of probiotics, which can regulate the ecological balance of animal intestinal microbial flora, promote the dominant growth of beneficial bacteria, and have the functions of preventing epidemic diseases and promoting growth. The analysis of relevant literature shows that there have been many literature reports on feed probiotics and feed additives at home and abroad. They are composed of different bacterial groups and are suitable for the breeding of poultry, livestock, fish and other animals. For example, the probiotic composition with Lactobacillus KE01 as the main component; the feed additive with Bacillus CIP5832 or enzyme as the main component; 6 ), actinomycetes, filamentous fungi or enzymes as the main ingredients; prebiotic 3000 composed of butyric aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K50/75
Inventor 张清敏崔合香崔小会
Owner NANKAI UNIV