Process for separating bacteria capable of degreding cellulose in straw

A separation method and cellulose technology, applied in bacteria and other directions, can solve the problems of low calorific value, environmental pollution, waste and so on

Inactive Publication Date: 2005-03-23
哈尔滨益生环境技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this way, the combustion is incomplete, the calorific value is low, and ser

Method used

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Experimental program
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specific Embodiment approach 1

[0004] Specific embodiment one: this embodiment is achieved in this way: a, enrichment and domestication: Pour the Hutchinson inorganic salt solid medium into a sterile plate, and put a piece of sterilized straw paper on it after the medium is solidified , and then use a sterile pipette to absorb the Hutchinson inorganic salt liquid medium to moisten the straw paper, take the sieved soil particles for spot planting, and place them in an incubator at 30°C to 50°C for 6 to 7 days of moisture cultivation. Pick the bacterial lawn around the soil particles with transparent circles, and use the flat-plate method to separate and purify the bacterial lawn; Shake on a rotating shaker for 2 to 5 hours, then carry out aseptic dilution to 1 to 10 bacteria / ml, draw the diluted bacteria solution onto the Hutcheson inorganic salt plate and shake well, then cover the plate with a sheet Sterile filter paper, 30 ℃ ~ 50 ℃ moisture culture for 5 ~ 7 days, the grown colonies are then diluted and c...

specific Embodiment approach 2

[0005] Specific implementation mode two: this implementation mode is realized in this way:

[0006] a. Enrichment and domestication: (1) air-dry the soil taken from Chengdu City, Sichuan Province, Qitaihe City, Heilongjiang Province, Northeast Agricultural University, Harbin Institute of Technology, take a 20-mesh sieve and a 40-mesh sieve, and put the 20-mesh sieve Put it on top, the two sieves fall together, pour the soil on the 20-mesh sieve to sieve the soil, take the soil in the middle of the two sieves for later use; (2) Weigh 1.0g KH 2 PO 4 , 0.1g CaCl 2 , 0.1g NaCl, 0.3g MgSO 4 ·7H 2 O, 2.5g NaNO 3 , 0.01g FeCl 3 , 18g of agar, then add 1000ml of distilled water, adjust pH=7.0~7.2, obtain the Hutchison inorganic salt solid medium at 121 DEG C of steam sterilization for 20min, the Hutchison inorganic salt liquid medium does not add agar, all the other formulas are the same; ( 3) Pour the above-mentioned Hutchinson inorganic salt solid medium into a sterile plate, ...

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PUM

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Abstract

The invention is a method for separating stalk cellulose-degrading bacteria, relating to a method of separating stalk cellulose-degrading bacteria from the soil. Its steps: a. pouring solid Hutchison inorganic salt culture medium into an axenic flat vessel, after the culture medium solidifying, placing a piece of sterilized coarse paper on the culture medium, then absorbing liquid Hutchison inorganic salt culture medium to wet the coarse paper, taking well-screened earth particles for point planting, and then placing them in a culture box to make moisture-retention culture, separating and purifying lawns by drawing lines on plate; b. putting the lawns in the physiological salt water containing glass beads for axenic diluting, absorbing the dilute bacterial solution on to the Hutchison inorganic salt plate and shaking the solution uniformly, covering a piece of axenic filter paper, and making moisture-retention culture; c. after primarily screening, inoculating each bacterial strain in a corresponding triangular bottle for liquid fermentation test, selecting out relatively high-sugar content bacteria from them all.

Description

Technical field: [0001] The invention is a method for isolating straw-like cellulose-degrading bacteria from soil. Background technique: [0002] Due to the rapid increase of the world's population and the utilization and destruction of limited energy by human beings, the development and utilization of new and renewable energy has attracted the attention of scientists all over the world. Cellulose is the most abundant available renewable organic resource on the earth's surface. The green plant cellulose raw material has a fast regeneration speed, and it accounts for more than 50% of the carbon content in the plant kingdom. Cellulose is the supporting tissue of plants and an important component of plant cell walls. It is a long chain connected by β-1.4 glucosidic bonds. Each cellulose molecule contains more than 10,000 glucose residues. Because cellulose has a water-insoluble, highly crystalline structure, and its periphery is surrounded by a lignin layer, it is difficult to...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 马放任南琪吴波远立江
Owner 哈尔滨益生环境技术有限公司
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