Chitinase gene of pea bean and its amino-acid squence of coded product

Abstract

The invention relates to a bean chitinase gene and the aminophenol sequence of its coded product, relating to a new gene sequence. It has a registered number AY357300 in GenBank, 1088bp long, where T, C, G and A are 232 (21.3%), 330 (30.3), 276 (25.4%) and 276 (23.0%), respectively, there is a starting codon ATG at 7bp site, there is a terminating codon TGA at 988bp site, and there is a poly (A) additional signal at 1008bp and 1082bp sites, respectively. It has no intron but complete 981bp-long open code read frame, coding 327 aminophenols. Its coded product has molecular weight of 35.3kD and equipotential point pI at 7.93. The invention separates a chitinase gene from bean genome, enlarges plant anti-fungal disease gene resources, thus providing richer excellent gene candidates for gene engineering of resisting plant diseases and insect pests and improving plant variety.

Description

Technical field: [0001] The present invention relates to a novel gene. Background technique: [0002] In recent years, fungal diseases have caused great harm to crops, so it is of great significance to prevent and control fungal diseases by means of genetic engineering. Since one of the cell wall components of many pathogenic fungi that harm plants is chitin, and no substrate for chitinase has been found in plants, chitinase plays an important role in defending against pathogenic fungi and is a plant defense important part of the system. Therefore, the isolation and transformation of chitinase gene has become a research hotspot in crop anti-fungal disease genetic engineering. [0003] The research on plant chitinase gene started in 1986. In 1986, Broglie et al. isolated the chitinase gene from kidney bean, with a reading frame of 984 nucleotides and encoding 328 amino acids. In 1991, Zhu et al. used the broad bean chitinase gene fragment as a probe to isolate the entire ...

AI Technical Summary

Problems solved by technology

[0004] At present, most of the chitinase genes are isolated from corn, rice, and tobacco, and there are few studies on the chitinase genes of kidney bean

And although the chitinase genes isolated in recent years have shown certain anti-fungal disease ability in transformed plants, it is still necessary to isolate chitinase genes with stronger anti-fungal disease ability.

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