Method for preparing lecithin in high purity

A phosphatidylcholine, high-purity technology, applied in the direction of edible phospholipid composition, food science, protein food ingredients, etc., can solve the problems of easily polluting the environment, affecting the application of phosphatidylcholine products, and difficult to completely remove acetonitrile solvent, etc. Achieve the effect of cleaning the production environment, improving labor efficiency and reducing production costs

Inactive Publication Date: 2005-06-15
HENAN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

Chinese Patent Publication No. CN1429828A patent discloses that the crude lecithin obtained after pretreatment with soybean oil foot is used as raw material, and silica gel is used as adsorbent, and methanol is used as eluent to carry out column chromatography separation to obtain high-purity lecithin; Phospholipids are refined with high-purity lecithin, and the elution is refined with methanol, which is easy to pollute the environment
Due to the use of highly toxic acetonitrile reagent as the extraction agent, it is difficult to completely remove the acetonitrile solvent in the phosphatidylcholine extract, which affects the application of phosphatidylcholine products

Method used

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  • Method for preparing lecithin in high purity

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Embodiment 1

[0016] Powder soybean lecithin raw material 20kg, add 100L of low-carbon alcohol-water mixed solvent (low-carbon alcohol: water is 90:10 volume ratio), mix and extract for 90 minutes, after centrifugation, add 100L low-carbon alcohol-water mixed solvent to the alcohol-insoluble matter , extracted for 60 minutes, and centrifuged. The alcohol-soluble matter was concentrated at a film centrifugal concentrator with a temperature of 70°C and a vacuum of 0.095mPa to obtain an alcohol concentrate. The mixed solvent of alcohol and water was used as the eluent for chromatographic separation. The eluent after chromatography obtained 4.62 kg of phosphatidylcholine product through vacuum concentration and drying for 240 minutes (75° C. of temperature, 0.096 Mpa of vacuum). The yield is 82.8%.

Embodiment 2

[0018] 10kg of powdered soybean lecithin raw material, add 20L of low-carbon alcohol-water mixed solvent (low-carbon alcohol:water is 98:2 volume ratio), extraction temperature is 30°C, mixed and extracted for 60 minutes, after centrifugation, add 30L to the alcohol-insoluble Low-carbon alcohol-water mixed solvent, extraction for 60 minutes, centrifugation, alcohol-soluble matter is concentrated in a thin-film centrifugal concentrator at a temperature of 75°C and a vacuum of 0.09MPa to obtain an alcohol concentrate, which is passed through an aluminum oxide chromatography column Chromatographic elution separation. The eluent after chromatography was concentrated and dried in vacuum for 210 minutes at a temperature of 80° C. and a vacuum of 0.096 MPa to obtain 3.05 kg of phosphatidylcholine product. The results of high-pressure liquid chromatography analysis showed that the content of phosphatidylcholine was 72.5 %, the product yield is 86.4%.

Embodiment 3

[0020] Powdered rapeseed oil phospholipid raw material 10kg, add 80L of low-carbon alcohol and water mixed solvent (low-carbon alcohol: water is 99:1 volume ratio), mix and extract at room temperature for 60 minutes, centrifuge, add 20L of low-carbon alcohol and water to the alcohol-insoluble matter and mix Solvent, extracted for 45 minutes, centrifuged, alcohol-soluble matter was concentrated in a thin film centrifugal concentrator with a temperature of 65°C and a vacuum of 0.095MPa to obtain an alcohol concentrate, which was separated by aluminum oxide chromatography column chromatography. The eluent after chromatography was concentrated and dried in vacuum for 240 minutes at a temperature of 75° C. and a vacuum of 0.098 MPa to obtain 2.55 kg of phosphatidylcholine product. The results of high-pressure liquid chromatography analysis showed that: phosphatidylcholine The content is 84.3%, and the product yield is 80.3%. .

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Abstract

A process for preparing the high-purity phosphatidylcholine from vegetative phosphatide includes extracting, separating alcohol-soluble components, vacuum centrifugal concentrating, adding it to chromatographic Al2O3 column, eluting with the mixture of water and low-carbon alcohol, vacuum concentrating and drying. Its advantages are high purity and quality, high output rate and no poison and pollution.

Description

(1) Technical field: [0001] The present invention relates to a kind of preparation method of chemical medicine product, especially the preparation method of high-purity phosphatidylcholine suitable for industries such as food, daily chemicals, health products and medicine, and it uses plant phospholipid (mainly soybean phospholipid) As raw materials, low-carbon alcohol and water are used as extraction agents, and high-purity phosphatidylcholine products are prepared by continuous extraction, centrifuge separation, centrifugal film vacuum concentration, column chromatography purification and vacuum drying. (2) Technical background [0002] Soy phospholipids are the by-products of soybean oil refining—the deeply processed products of hydrated oil feet, mainly including phosphatidylcholine (PC), phosphatidylcholine (PE), phosphatidic acid (PA), phosphatidylinositol (PI), Composed of sugar esters and glycerides. Phosphatidylcholine is an excellent natural emulsifier and nutriti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10
Inventor 李桂华张根旺高风海姜延超谷克仁李晓洁
Owner HENAN UNIVERSITY OF TECHNOLOGY
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