Method for preparing acanthopanax injectio

A technology of injection preparations and Acanthopanax, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, powder delivery, etc., and can solve the problems of low active ingredient content, time-consuming, and inability to extract

Inactive Publication Date: 2005-07-06
TIANJIN TASLY ZHIJIAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The weak point of this technique is that adopting 75% ethanol can not extract the polysaccharide active components that Acanthopanax senticosus has to improve immunity, because the polysaccharide components are insoluble in 75% ethanol, in addition, to adopting 75% to extract The effective component that goes out is also without purification process, makes the content of active component wherein not high, therefore, this is also the problem to be solved by the method of the present invention
Chinese patent CN1053817C reports the preparation method of Acanthopanax anticanax extract and the injection containing the extract and freeze-dried powder injection, and its preparation process also adopts macroporous adsorption resin, but it only mentions the use of macroporous resin for decolorization, and The specific test method was not specified, and the reported Acanthopanax injection and freeze-dried powder did not retain pharmacologically active polysaccharide components, because the extraction method used was also the traditional water extraction and alcohol precipitation process , water extraction can extract polysaccharides, but alcohol precipitation process will remove polysaccharides
Chinese patent CN00123053 also discloses the production process of Acanthopanax senticosus essence extract, which uses 50%-80% ethanol to leach first, then heats and extracts with 50%-80% ethanol, and then passes through a macroporous adsorption resin column. Before applying the macroporous adsorption resin, the solution is clarified by adding water and standing for refrigeration, so as to pre

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take Acanthopanax and crush it into granules, reflux extraction twice, add 8 times the amount of 70% ethanol (v / v) for reflux extraction each time, filter, and keep the dregs for later use; combine the filtrates, concentrate under reduced pressure until there is no alcohol smell , add distilled water so that every 1ml solution is equivalent to 1g of crude drug, centrifuge at high speed, add the supernatant to the processed D 101 On the macroporous adsorption resin column, remove impurities by first eluting with water, then eluting with 20% ethanol, collecting the 20% ethanol eluate, recovering the ethanol under reduced pressure to dryness, and obtaining the total glycosides component of Acanthopanax senticosus; The Acanthopanax senticosus dregs after alcohol extraction is heated and extracted three times with water, the water extracts are combined, concentrated, repeated alcohol precipitation three times, the precipitate is added with water to dissolve, and made into a 1...

Embodiment 2

[0044] Take Acanthopanax and crush it into granules, reflux extraction twice, add 10 times the amount of 50% ethanol (v / v) for reflux extraction each time, filter, keep the dregs for later use; combine the filtrates, concentrate under reduced pressure until no alcohol smell , add distilled water so that every 1ml solution is equivalent to 1g crude drug, centrifuge at high speed, add the supernatant to the AB-8 macroporous adsorption resin column that has been processed, first wash with water to remove impurities, and then use 40% ethanol to elute. Collect 40% ethanol eluted parts, recover ethanol under reduced pressure to dryness, and obtain the total glycoside components of Acanthopanax senticosus; extract the dregs of Acanthopanax senticosus after alcohol extraction by heating with water three times, combine the water extracts, concentrate, and repeat Precipitate with alcohol three times, add water to dissolve the precipitate, make a 1% solution, pass through a hollow fiber c...

Embodiment 3

[0050] Take Acanthopanax and crush it into granules, reflux extraction twice, add 10 times the amount of 85% ethanol (v / v) for reflux extraction each time, filter, and keep the dregs for later use; combine the filtrates, concentrate under reduced pressure until there is no alcohol smell , add distilled water so that every 1ml solution is equivalent to 1g of crude drug, centrifuge at high speed, add the supernatant to the AB-8 macroporous adsorption resin column that has been processed, first wash with water to remove impurities, and then use 70% ethanol to elute, Collect 70% of the ethanol eluted part, recover ethanol under reduced pressure to dryness, and obtain the total glycoside components of Acanthopanax senticosus; extract the dregs of Acanthopanax senticosus after alcohol extraction by heating with water three times, combine the water extracts, concentrate, and repeat Precipitate with alcohol three times, add water to dissolve the precipitate, make a 1% solution, pass th...

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PUM

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Abstract

The invention discloses a method for preparing acanthopanax injection, which consists of employing alcohol and water extraction method to extract the glycosides components and polysaccharides active constituents in acanthopanax root, refining glycosides components through macroscopic adsorption resin column purifying, removing impurities from polysaccharides component through water extraction alcohol depositing method, removing small molecules through hollow fiber column hyperconcentration, the effective component with activity in the acanthopanax root can be fully preserved.

Description

[0001] Field [0002] The invention belongs to the field of traditional Chinese medicine pharmacy, and in particular relates to a method for preparing Acanthopanax senticosus injection preparation. Background technique [0003] Acanthopanax is the dried root, rhizome or stem of Acanthopanax acanthopanax, which has the effects of nourishing Qi, invigorating the spleen, tonifying the kidney and calming the nerves. Acanthopanax contains active ingredients such as eleutherosides A, B, C, D, E, isoferidin and water-soluble acanthopanax polysaccharides. According to literature reports, Acanthopanax has strong effects of promoting blood circulation and removing blood stasis, dredging meridians and activating collaterals, can dilate blood vessels, improve blood flow in heart, brain, kidney and other substantial organs, and increase tissue tolerance to hypoxia. Can significantly remove reactive oxygen species, reduce platelet aggregation, dilate coronary arteries, improve myocardial m...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61P9/10A61P15/12
Inventor 张晴龙
Owner TIANJIN TASLY ZHIJIAO PHARMA
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