Animal interferon gene engineering biologically active preparation
A genetic engineering and biologically active technology, applied in the field of biological compositions, can solve problems such as product contamination, vaccines that are difficult to completely control or purify viruses, and animal health contamination.
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[0010] The present invention uses genetic engineering interferon and its inducing factor interleukin 18 as raw materials. We cloned and purified the interferon genes of chicken, pig, and cattle, recombined them into plasmids respectively, and introduced them into the genetically engineered yeasts, and then the engineered bacteria were fermented and proliferated to express a large amount of interferon gene products, which is genetic engineering interference Through high-efficiency expression and interferon coating regulation technology, three new genetically engineered interferon drug products with biological activity in chicken, pig and cattle have been successfully developed. Then the antiviral titers were tested with chicken embryo fibroblasts and pig PK cells, and their vigor reached 5×10. 5 Activity unit / ml or more.
[0011] The proportion of each component according to the biological activity titer is as follows:
[0012] Genetic engineering interferon 5×10 5 Active unit / ml, ...
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