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Animal interferon gene engineering biologically active preparation

A genetic engineering and biologically active technology, applied in the field of biological compositions, can solve problems such as product contamination, vaccines that are difficult to completely control or purify viruses, and animal health contamination.

Inactive Publication Date: 2005-07-06
山东华辰制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially in recent years, the continuous occurrence of immunosuppressive virus infection, the continuous mutation of bird flu and other viruses, resulting in poor immune effect of animal vaccines, atypical Newcastle disease and atypical swine fever occur frequently, and the phenomenon of animal health infection is very serious
This makes problems such as recessive source of infection, fauna biological contamination and product poisoning unresolved, and so far there is no class of compound drugs that can effectively kill livestock viruses and effectively control the occurrence of chicken viral infectious diseases
The production practice of the breeding industry shows that it is difficult to completely control or purify the virus pollution to the herd with the vaccine alone, especially in recent years, the pollution of the immunosuppressive virus has been increasing, which makes the vaccination often fail

Method used

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Embodiment Construction

[0010] The present invention uses genetic engineering interferon and its inducing factor interleukin 18 as raw materials. We cloned and purified the interferon genes of chicken, pig, and cattle, recombined them into plasmids respectively, and introduced them into the genetically engineered yeasts, and then the engineered bacteria were fermented and proliferated to express a large amount of interferon gene products, which is genetic engineering interference Through high-efficiency expression and interferon coating regulation technology, three new genetically engineered interferon drug products with biological activity in chicken, pig and cattle have been successfully developed. Then the antiviral titers were tested with chicken embryo fibroblasts and pig PK cells, and their vigor reached 5×10. 5 Activity unit / ml or more.

[0011] The proportion of each component according to the biological activity titer is as follows:

[0012] Genetic engineering interferon 5×10 5 Active unit / ml, ...

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PUM

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Abstract

The invention concerns a gene engineering biological active formulation of animal interferon prepared by making gene engineering interferon, its induction factor interlukin 18 and coating regulating factor as materials. The ingredients ratio in biological active potency is as follows: gene engineering interferon 5í‡105 active unit / ml, interlukin 18í‡102 active unit / ml, coating regulating factor 10 active unit / ml. The invention can considerably enhance immune capability of animal body, rapidly and effectively eradicate invasive virus inside the animal body.

Description

Technical field [0001] The invention relates to the technical field of biological compositions for killing viruses in animals, in particular to an animal interferon genetic engineering bioactive preparation. Background technique [0002] The continuous mutation of viruses such as animal health and avian influenza, atypical Newcastle disease, atypical swine fever, etc., have caused the long-term existence of viral infectious diseases in the animal population or the biological contamination of their products, which has always troubled and restricted my country's animal breeding industry. One of the key issues in the competitiveness of livestock products in the international market. Especially in recent years, the continuous occurrence of immunosuppressive virus infections and the continuous mutation of viruses such as avian influenza have caused poor immune effects of animal vaccines. Atypical Newcastle disease and atypical swine fever often occur, and the phenomenon of animal heal...

Claims

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Application Information

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IPC IPC(8): A61K38/21A61P31/12C12N15/20
Inventor 姜正军赵宏坤陈溥言曹瑞兵刘刚王春民胡敬东张西雷
Owner 山东华辰制药有限公司