Unlock instant, AI-driven research and patent intelligence for your innovation.

Rat brain division tissue RNA molecular film, and its preparation method and kit

A technology of ribonucleic acid and partitioned organization, which is applied in the field of mouse brain partitioned ribonucleic acid molecular membranes and its preparation and kits, can solve the problems of difficult ribonucleic acid separation, research hindrances, and difficulty in obtaining it, and achieves convenience in use and reduced research costs. The effect of cost and strength enhancement

Inactive Publication Date: 2005-07-06
GUANGDONG INST OF MICROORGANISM
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many of these studies cannot be directly conducted with human brain tissue, and need to be conducted with the brain tissue of experimental animals, among which mice and rats are one of the most commonly used experimental animals
Due to the difficulty of obtaining experimental materials (brain subregions) and the difficulty of separating RNA, many related research work in the laboratory have been hindered

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rat brain division tissue RNA molecular film, and its preparation method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 14 kinds of brain tissues were taken from mice (BALB / C strain, clean grade): anterior cortex, posterior cortex, hippocampus, olfactory bulb, striatum, thalamus, cerebellum, midbrain, medulla oblongata, pons, tonsil, pituitary gland, pineal gland, Whole brain, according to the "one-step method" (specific operation according to the literature Chomzynski P, Sacchi N. Single-step methoded of RNA isolation by acid guanidiniumthiocyanatr-phonol-chloroform extraction. Anal. Biochem., 1987, 162: 156-159) to isolate the total Ribonucleic acid, detected by formaldehyde denatured agarose gel electrophoresis, 18S ribosomal ribonucleic acid band and 28S ribosomal ribonucleic acid band are clear, and the brightness ratio of the two is 1:2; then use Oligo(dT)-cellulose affinity layer Messenger ribonucleic acid is isolated from total ribonucleic acid by analysis method (specific operation is by document Sambroook J, etal. Molecular Cloning: A Laboratory Manual. 2nd ed. New York: Cold Sp...

Embodiment 2

[0046] Take the qualified mouse brain partition tissue ribonucleic acid molecular membrane product (1 sheet) of Example 1, and package it into a kit together with additional hybridization solution (30mL) and β-actin cDNA control probe (100ng).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
quality scoreaaaaaaaaaa
Login to View More

Abstract

This invention relates to a mouse brain subarea structure plasmonucleic acid molecule filme and its process method and agent case, which is characterized by the following: separately collecting subarea structure and total brain structure; getting the film with marked sample holes and plasmonucleic molecule mass positions through total plasmonucleic acid isolation, messenger plasmonucleic isolation, modified agar gel electrophoresis, film transferring and fixing. The agent case comprises the molecule film and cross liquid and cDNA comparing needles.

Description

technical field [0001] The invention relates to a ribonucleic acid molecular film of rat brain partition tissue, a preparation method and a kit thereof. Background technique [0002] In molecular biology research, a ribonucleic acid (RNA) hybridization analysis technique called Northern Blot is often used to detect gene expression in tissues or cells. Through this ribonucleic acid hybridization experiment, the expression of specific genes or new genes in tissues and cells can be identified; the abundance of gene expression, transcript size and splicing mode can be detected; gene homology and expression differences between different tissues and cells can be compared Wait. [0003] With the continuous progress of molecular biology research, ribonucleic acid hybridization technology has also been continuously improved and developed. At first, ribonucleic acid was immobilized on unstable diazotized cellulose membrane or fragile nitrocellulose membrane. It is now fixed on a mor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01D67/00B01D69/02C07H1/06C12Q1/68
Inventor 叶志华郭俊梁建庆
Owner GUANGDONG INST OF MICROORGANISM