Process for extracting starfish steroid glycoside from starfish

A technology of steroidal saponins and starfish, which is applied in the field of extracting starfish steroidal saponins, can solve the problems of unsuitable extraction of large polar or macromolecular substances, unsatisfactory extraction effect, low dielectric constant, etc., and achieve good selectivity, The effect of shortening the processing time and maintaining biological activity

Inactive Publication Date: 2005-07-06
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
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Problems solved by technology

[0005] Must see, due to SC-CO 2 The properties of weak van der Waals force and low dielectric constant are not suitable for the extraction of more polar or macromolecular substances, wh

Method used

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  • Process for extracting starfish steroid glycoside from starfish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh 200g starfish dry powder into the extraction tank, use CO 2 As a supercritical fluid, the extraction pressure is 25MPa, and the extraction temperature is 323K. One-stage separation is adopted, the pressure of the separation column is 5MPa, the temperature of the separation column is 333K, and the extraction time is 3h. The extract was discharged from the separator, weighed, and the yield of total extract was calculated. The content of starfish saponin in the extract was determined by ultraviolet-visible spectrophotometry, and the yield was calculated. It is determined that the yield of the total extract is 1.98%, and starfish saponin is basically not extracted.

[0025] This example illustrates pure supercritical CO 2 Only the fat-soluble part of starfish can be extracted, but not the more polar saponins.

Embodiment 2

[0027] Weigh 200g starfish dry powder and put it into the extraction tank, and degrease according to the method of implementation case 1. The extraction temperature was 323K, the pressure was 25MPa, methanol and 95% ethanol were used as co-solvents respectively, 200mL of co-solvent was added first, static extraction was carried out for 0.5h, 100mL of co-solvent was added by pump after 1h, and 100mL of co-solvent was added by pump after 1h. All the other conditions and methods are the same as in Example 1. The extraction results are shown in Table 1.

[0028] Table 1 Effects of different types of cosolvents on the extraction of starfish saponins

[0029] Total Extract Yield Starfish Saponin Yield Starfish in Total Extract

[0030] Co-solvent type

[0031] (%) (%) Saponin content

[0032] Ethanol 2.18 0.59 27.06

[0033] Methanol 2.25 0.62 27.56

[0034] The purpose of degreasing according to Example 1 is to reduce the interference of...

Embodiment 3

[0036] Weigh 200g starfish dry powder and put it into the extraction tank, and degrease according to the method of implementation case 1. Prepare 0.01mol / L surfactant-ethanol solution of AOT, TX-10, CTBA, Span, AGP, Tween as a co-solvent, add 200mL to the extractor, and extract statically for 3h at a temperature of 323K and a pressure of 25MPa, according to the implementation The method of example 1 calculates the yield of total extract, starfish saponin, and the content of starfish saponin in the extract, and the results are shown in Table 2.

[0037] Table 2 Effects of different types of surfactants on the extraction of starfish saponins

[0038] Total Extract Yield Starfish Saponin Yield Starfish Saponin in Total Extract

[0039] Surfactant

[0040] (%) (%) Glycoside content (%)

[0041] None 1.06 0.25 23.58

[0042] AOT 1.32 0.36 27.27

[0043] TX-10 1.25 0.31 24.80

[0044] CTBA 1.22 0.29 23.77

[0045] Span 1.09 0.25 22.94

[00...

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Abstract

The invention discloses a process for extracting a starfish steroid saponin from starfishes. The technical characteristic is that polyalcohol and water mixed solution with a dissolved surfactant is used as a lacquersolvent for intensifying the process of extracting the starfish saponin using supercritical CO2. By the process, the prepared starfish saponin is stable in quality and easy for control, and the physiological activity of the saponin can be kept.

Description

technical field [0001] The invention relates to a method for extracting starfish steroidal saponins from starfish, specifically a method using supercritical CO 2 The method of enhanced extraction is the method of extracting starfish steroidal saponins from starfish. Background technique [0002] The abuse of synthetic antibiotics and hormonal drugs has led to the continuous increase of bacterial resistance in the human body, organisms, and even the entire ecosystem, which has brought major hidden dangers to the safety of the ecological environment and made human beings face severe health challenges. Therefore, preparing natural active substances with unique activity and broad-spectrum antibacterial properties from natural products to develop safe and efficient natural antiviral drugs is of great significance for maintaining human health and ecological environment safety. [0003] Starfish is a common marine organism in my country's sea areas. In recent years, a variety of s...

Claims

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Application Information

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IPC IPC(8): B01D11/04C07J75/00
CPCY02P20/54
Inventor 党志文震朱志鑫
Owner SOUTH CHINA UNIV OF TECH
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