Biodegradable calcium phosphate/collagen composite materials for medical use and method for preparation thereof

[0023] Example 1

[0024] Dissolve 0.2g of collagen in 400ml of 0.01M hydrochloric acid solution while stirring with a magnetic stirrer. After the milky white solution is formed, add 0.5g of α-phase tricalcium phosphate/β-phase tricalcium phosphate composite powder into it evenly. The α-phase phosphoric acid in the composite powder The ratio of tricalcium to β-phase tricalcium phosphate is 1:3, then add 20ml of 0.1% glutaraldehyde solution and continue to stir slowly until white flocculent suspended matter appears. After it is completely deposited, add KOH solution to pH 7. After standing for 48 hours, the supernatant was removed. The obtained material was placed in a plastic mold with micropores on the surface and cooled in liquid nitrogen for 60 minutes, and then placed in a freeze dryer to dry for 96 hours. After the material was taken out, it was soaked in ethanol for 24 hours × 3 times, and again Put it into a freeze dryer to dry for 60 hours, and then sterilize it in ethylene oxide steam for 1 hour. After the product of the present invention is quenched by liquid nitrogen, its section is detected by scanning electron microscope, and the internal appearance of the product is as figure 1 As shown, it can be seen from the figure that the internal pores of the prepared medical calcium phosphate/collagen composite material are connected.

[0025] Example 2

[0026] Dissolve 0.4g collagen in 100ml 0.5M acetic acid solution while stirring with a magnetic stirrer. After it is dissolved, add 1.0g amorphous calcium phosphate powder to the collagen solution, and then add 8ml 0.5% glutaraldehyde solution. Stir continuously until white flocculent suspended matter appears, add NH after it is completely deposited 4 The OH solution reaches the pH value of 7, and the supernatant is removed after standing for 36 hours. The obtained material is placed in a plastic mold with micropores on the surface and cooled in liquid nitrogen for 30 minutes, and then placed in a freeze dryer to dry for 72 hours. After the material is taken out, it is placed in ethanol Soak in the medium for 24h×3 times, put it in a freeze dryer again to dry for 48h, take it out, and sterilize it in ethylene oxide steam for 3h.

[0027] Example 3

[0028] 1.5g of carbonate-containing hydroxyapatite/α-phase tricalcium phosphate powder is evenly added to 200ml of collagen solution with a concentration of 3.0mg/ml. The ratio of carbonate-containing hydroxyapatite and α-phase tricalcium phosphate in the composite powder is 1:1. After stirring evenly, add 6ml of 1% glutaraldehyde solution and continue stirring until white flocculent suspended matter appears. After it is completely deposited, add NaOH solution to pH 6, and let it stand for 24h before removing the supernatant. Put the resulting material on the surface The microporous plastic mold is cooled in liquid nitrogen for 10 minutes, and then placed in a freeze dryer to dry for 72 hours. After the material is taken out, it is soaked in ethanol for 24 hours × 3 times, and then placed in the freeze dryer to dry for 48 hours. Sterilize in oxyethane steam for 2h.