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Preparation of chitin oligose compound

A technology of chitooligosaccharides and compounds, which is applied in the field of preparation of chitooligosaccharides, can solve the problems of reducing the action point of degradation, prolonging the reaction time, and reducing the reaction speed, so as to achieve short degradation reaction time, reduce losses and Effects of waste and productivity improvement

Inactive Publication Date: 2005-10-12
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under acidic conditions, when the molecular weight of chitosan reaches about 8000, chitosan reaches water solubility, and excessive hydrogen ions form NH with the amino groups of chitosan molecules. 4 + , on the contrary, it reduces the action point of degradation, reduces the reaction speed and prolongs the reaction time, resulting in waste of time and economic loss

Method used

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  • Preparation of chitin oligose compound
  • Preparation of chitin oligose compound
  • Preparation of chitin oligose compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Weigh an appropriate amount of polysaccharide powder of the shell, dissolve it into a viscous solution with 15 times its weight and 4% (V / V) acetic acid, stir evenly, keep the temperature at 50°C, and add a concentration of 30% (V / V ) hydrogen peroxide solution, make R=1, react for 2 hours, then add 10mol / L potassium hydroxide solution, adjust the pH value of the reaction medium to 7, continue to react for 1 hour, cool to room temperature, and use solution volume 3 times Precipitate with ethanol, centrifuge, and dry the obtained precipitate at 40°C to obtain the chitooligosaccharide compound. Grind the dried product to 20 mesh, and measure its molecular weight with an Ubbelohde viscometer to be 8.70×10 3 , and its yield was 62.1%.

Embodiment 2

[0021] Take an appropriate amount of chitosan powder, dissolve it into a viscous solution with 20 times its weight and 5% (V / V) acetic acid, stir evenly, and add a concentration of 30% (V / V) at a constant temperature of 60°C. ) hydrogen peroxide solution, make R=1, react for 4 hours, then add 8mol / L sodium hydroxide solution, adjust the pH value of the reaction medium to be 8, continue to react for 2 hours, after the reaction is completed, part of the water is evaporated under reduced pressure , the volume of the solution is concentrated to about 40% of the original volume, cooled to room temperature, precipitated with ethanol 5 times the volume of the remaining solution, centrifuged, and the resulting precipitate is vacuum-dried at room temperature to obtain the chitooligosaccharide compound. Grind the dried product to 60 mesh, and measure its molecular weight with an Ubbelohde viscometer to be 9.74×10 3 , and its yield was 61%.

Embodiment 3

[0023] Take an appropriate amount of chitosan powder, dissolve it into a viscous solution with hydrochloric acid 25 times its weight and a concentration of 3% (V / V), stir evenly, keep the temperature at 80°C, and add a concentration of 30% (V / V) ) hydrogen peroxide solution, make R=1, react for 6 hours, then add 5mol / L sodium hydroxide solution, adjust the pH value of the reaction medium to be 10, continue to react for 3 hours, after the reaction is completed, part of the water is evaporated under reduced pressure , the volume of the solution was concentrated to about 60% of the original volume, cooled to room temperature, precipitated with ethanol 10 times the volume of the remaining solution, centrifuged, and the resulting precipitate was dried at 60°C to obtain the chitooligosaccharide compound. Grind the dried product to 100 mesh, and measure its molecular weight with an Ubbelohde viscometer to be 4.48×10 3 , and its yield was 38.1%.

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Abstract

The invention is about the process to produce the chitose. The process is : first to dissolve the chitosan in the acid and add into the H2O2 at 50-80deg.C for 2h, next to add into the alkali to adjust pH7-10 for 1-3h, last to add into the ethanol of 3-10 times volumes to deposit the chitose. The process has the good efficiency and short reaction time.

Description

technical field [0001] The invention relates to the preparation of chitooligosaccharides, in particular to a method for preparing chitooligosaccharide compounds. Background technique [0002] Oligosaccharides and oligosaccharides have a variety of biological functions. Studies have proved that they are widely used in anticancer drugs, diagnostic reagents, plant physiology, food, cosmetics, etc., and their development and application prospects are very broad. Moreover, compared with chitin and chitosan, it has very prominent advantages, such as being easily soluble in water, non-antigenic, and they have a weak accumulation effect in the host body. However, the research on the preparation technology and mechanism of action of oligosaccharides and oligosaccharides is not yet clear, and the yield of the preparation process is low, making them very expensive. Therefore, the large-scale industrial application of oligosaccharides and oligosaccharides is largely restricted. The ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 李鹏程郭占勇刘松刑荣娥于华华王丕波
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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