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Method for producing puerarin in high purity and aglycon of soybean jointly

A technology for joint production of daidzein, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of difficult industrial production, unsafe use, high cost, etc., and achieve good product quality and low cost , the effect of high content of active ingredients

Inactive Publication Date: 2005-11-16
何照范
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method adopts solvent ethanol or methanol extraction, S-8 resin adsorption, solvent elution, and then carries out or handles, and this method needs to use adsorption resin, and cost is higher; Use poisonous methanol as solvent, use unsafe; And adopt column analysis It is difficult to realize industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] The first step is to take 5kg of fresh kudzu root, wash it, cut it into short pieces, and break it into pieces;

[0010] In the second step, add 20 to 25 kg of 60% to 80% ethanol to the crushed product, heat to 50 to 60°C, reflux and extract for 4 hours, then cool to room temperature, separate and remove the dregs with a centrifuge, and then concentrate the extract into extract;

[0011] In the third step, add 5% dilute hydrochloric acid to heat and hydrolyze for 8 hours, filter while hot, filter out the acid solution, add ethyl acetate to extract 3 times after cooling, and then combine the 3 extracts;

[0012] The fourth step is to take the extracted acid solution for heating, evaporating and concentrating, then cooling, standing for crystallization, suction filtration to remove the mother liquor, and then recrystallizing three times with pure water or 20% to 40% ethanol, and drying with hot air. 15.5 g of puerarin crystals were obtained with a purity of 98.5%;

[00...

Embodiment 2

[0015] In the first step, take 1 kg of kudzu root dry slices and coarsely crush them;

[0016] In the second step, add 10 to 15 kg of 60% to 80% ethanol to the crushed product, heat it to 70 to 80 ° C, reflux and extract for 9 hours, then cool to room temperature, separate and remove the dregs with a centrifuge, and then concentrate the extract into extract;

[0017] In the third step, add 8% dilute hydrochloric acid and heat and hydrolyze for 10 hours, filter while hot, filter out the acid solution, add ethyl acetate after cooling to extract 3 times, and then combine the 3 extracts;

[0018] The fourth step is to take the extracted acid solution for heating, evaporating and concentrating, then cooling, standing for crystallization, suction filtration to remove the mother liquor, and then recrystallizing three times with pure water or 20% to 40% ethanol, and drying with hot air. 13.6g of puerarin crystals were obtained with a purity of 97.5%;

[0019] The fifth step is to ta...

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PUM

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Abstract

A process for preparing high-purity kakonein and daidzin from pueraria root includes mixing puerania root with the aqueous solution of ethanol, heating while reflux extracting, centrifugal separation to emove dregs, concentrating, acidolyzing, extracting active component by ethyl acetate, concentrating for crystallizing, filtering to separate acidic liquid, recrystallizing in purified water or alcohol, drying to obtain high-purity daidzin, concentrating separated acidic liquid, crystallizing, filtering, recrystallizing in purified water or alcohol, and drying to obtain high-purity kakonein.

Description

technical field [0001] The invention relates to a method for jointly extracting active ingredients from traditional Chinese medicines, in particular to a method for jointly extracting puerarin and daidzein from kudzu root. Background technique [0002] Pueraria lobata is the tuber of the leguminous plant Pueraria lobata. It is sweet and pungent in taste and flat in nature. It is used as medicine because it contains isoflavones. In recent years, people have continued to study the method of extracting the active ingredients of Pueraria lobata. After discovering that daidzein, one of the effective bioactive ingredients in Puerarin isoflavones, has stronger biological activity, the research on how to extract the active ingredients of Pueraria lobata becomes more important. At present, there are many methods for extracting puerarin flavonoids or extracting puerarin, but there are few patent literature reports on extracting daidzein. Through searching, only Chinese patent applicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07
Inventor 何照范何珺
Owner 何照范
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