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Portable instrument for detecting pesticide residue through fluorescent probe

A fluorescent probe, pesticide residue technology, applied in fluorescence/phosphorescence, sample preparation for testing, material excitation analysis, etc. The effect of low matrix interference and easy operation

Inactive Publication Date: 2006-02-01
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

In addition, the rapid measuring instrument using the chromogenic method is susceptible to the interference of chlorophyll and other substrates in fruits and vegetables, and the color development is often not obvious, which makes it difficult to judge the results, resulting in missed detection.

Method used

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  • Portable instrument for detecting pesticide residue through fluorescent probe
  • Portable instrument for detecting pesticide residue through fluorescent probe

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Embodiment Construction

[0032] The principle of the present invention is that organophosphorus pesticides and carbamate pesticides can be combined with the hydroxyl group on serine in the active center of cholinesterase to phosphorylate or carbamoylate cholinesterase, preventing the substrate acetylcholine and choline The combination of alkaline lipase, thereby inhibiting the enzyme activity, reducing the rate of the enzyme-catalyzed substrate reaction, thereby reducing the rate of acetic acid generation, resulting in a decrease in the pH value change; due to the different fluorescence intensities of the coexisting fluorescent probes at different pH values, the pH The change rate of fluorescence intensity caused by different value changes is also different. By detecting the change rate of fluorescence intensity, the amount of pesticide residues can be determined indirectly.

[0033] Such as figure 1 As shown, the portable fluorescent probe pesticide residue detector of the present invention is mainly...

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Abstract

A portable fluorescent probe detector of pesticide residue is composed of optical unit, detection and data processing system as well as power unit. it is featured as forming optical unit by light source, filter, detector and photoelectric tube; setting excitation light filter and photoelectric tube in sequence on light source light path; setting fluorescent filter between excitation light filter and photoelectric tube; setting detector on coaxial after fluorescent filter; connecting detector to amplifier, power to light source and detector; Data processing unit to amplifier and photoelectric tube as well as to display.

Description

technical field [0001] The patent of the invention relates to the technical field of trace detection, which is a detection instrument for pesticide residues in vegetables and fruits, especially a portable instrument based on fluorescent probe technology for on-site detection of organic phosphorus and carbamate pesticide residues in vegetables and fruits instrument. Background technique [0002] The large-scale use and abuse of pesticides for a long time has made the phenomenon of pesticide residues in agricultural products such as vegetables and fruits quite serious in China. In addition, most of the agricultural products such as vegetables and fruits in my country are grown by individual households, so the excessive use and abuse of pesticides is difficult to control. With the improvement of people's food safety requirements, there is an urgent need to develop a detection instrument for pesticide residues in fruits and vegetables that is moderately priced, has high detectio...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/28
Inventor 陈吉平张纪秀车迅黄威东郭荣波
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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