Method for cultivating selenium-enriched officinal dendrobium stem
A technology of Dendrobium officinale and a cultivation method, which are applied in the directions of horticultural methods, botanical equipment and methods, plant phenotype improvement, etc., can solve the problems of difficulty in artificial cultivation of Dendrobium officinale, selenium elements affecting the growth of Dendrobium officinale, and different cultivation methods, etc. The production process is easy to operate, convenient for large-scale production, and the dry matter weight is improved.
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[0023] Embodiment: the cultivation method of selenium-enriched Dendrobium officinale
[0024] Adopt the method of tissue culture, get Dendrobium officinale seed aseptic test-tube seedling induction, protocorm induction, protocorm acclimatization, then transfer to growth on the medium, because the above-mentioned tissue culture technology of Dendrobium candidum is existing mature technology, it is ominous to describe here, For the configuration of the culture medium, its specific method steps are as follows:
[0025] Culture solution configuration:
[0026] 1), inorganic nutrients:
[0027] Trace elements (1000 ml, 1000 ml can be prepared by absorbing 1 ml when preparing the medium): 0.8326 grams of potassium iodide (KI); 0.2532 grams of sodium permanganate (NaMnO4.2H2O); 0.0248 grams of copper sulfate; 0.0256 grams of cobalt chloride 22.3212 grams of manganese sulfate; 8.6281 grams of zinc sulfate; 6.2308 grams of boric acid macroelements (in 1000 milliliters, the following ...
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