Method for obtaining Tibetan Sinopodophyllum hexandrum hairy root which produce podophyllotoxin through agrobacterium rhizogenes genetic transformation, and its product

Abstract

The invention provides a method of producing hair roots of Tibet peaches for podophyllinic acid lactone by gene engineering to convert Tibet peache genes. The invention relates to a method of converting Tibet peache genes with hair root agricillins to gain hair roots of Tibet peaches for podophyllinic acid lactone. Its process is: convert Tibet peache genes with hair root agricillins to gain hair roots of Tibet peaches for podophyllinic acid lactone, ascertain said hair roots by molecule detection, and the results of HPLC detection show that hair roots of Tibet peaches produced by gene convertion could be used to produce podophyllinic acid lactone. The invention offers a lasting new pattern drug-source to the production of podophyllinic acid lactone.

Description

technical field

[0001] The invention belongs to the fields of molecular biology, physiology, breeding, genetic engineering and the like, and relates to a method for obtaining podophyllotoxin-producing Tibetan peach seven hairy roots by using transgenic technology, in particular to the genetic transformation of Tibet peach seven by Agrobacterium rhizogenes And obtain the specific process of producing podophyllotoxin-like seven-haired root of Tibetan peach. The present invention also provides podophyllotoxin-producing Tibetan chinensis root obtained by genetic engineering and its cultured progeny, regenerated plant, plant tissue or seed. Background technique

[0002] Plant secondary metabolites have extremely complex chemical structures, and no effective or economical synthesis methods have been found so far. Compared with conventional cell culture, the hairy root culture system has the advantages of rapid growth, no need for exogenous plant hormones, strong and stable abilit...