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Comprehensive extraction process of active components in fresh pilose antler

A technology of active ingredients and comprehensive extraction, applied in the field of extracting active ingredients from velvet antler, can solve the problems of ignoring the utilization of active ingredients, destroying the activity of heat-sensitive ingredients, reducing the application value of velvet antler, and achieving the effect of improving the application value

Inactive Publication Date: 2006-05-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the traditional processing of velvet antler, velvet antler has to go through steps such as boiling, frying, and drying. Due to repeated long-term high-temperature cooking, many water-soluble components will be lost on the one hand, and heat-sensitive components such as proteins and peptides will be lost. The activity is completely destroyed
Therefore, the traditional processing technology can not make full use of the active ingredients in velvet, which greatly reduces the application value of velvet and causes a lot of waste of resources
[0004] Some patents have introduced the research of extracting growth factors from velvet antler (China, CN 1104095A, 1995; China, CN 1603344A, 2005), but these methods ignore the utilization of active ingredients other than proteins when extracting polypeptides in velvet velvet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 11000

[0014] Example 11000 g of fresh velvet antler is singeed, washed, cut into pieces, and crushed to a size of 120-350 μm by a colloid mill. The crushed fresh velvet antler is firstly pre-frozen at -20°C, and then dried on a freeze dryer at -30°C to -50°C, and the moisture content of the dried product is less than 5%.

[0015] Put 200g of deer antler freeze-dried powder into the extraction kettle, the extraction temperature is 35°C, the extraction pressure is 55MPa, CO 2 The flow rate is 15kg / h; the pressure of the primary separation tank is 15MPa, the temperature is 35°C, the extract is light yellow paste, and the extraction rate is 1.5-2.0%, which contains estradiol, estriol, progesterone and testosterone , lower fatty acids, phospholipids, and sitosterol; the pressure of the secondary separation tank is 5MPa, the temperature is 50°C, the extract is milky white oily, the extraction rate is 1.0-1.5%, and it contains linoleic acid, linolenic acid, di Active ingredients such as s...

Embodiment 2

[0017] Embodiment 2 adopts extraction temperature 35 ℃, extraction pressure 20MPa, other supercritical CO 2 The extraction and separation process conditions are the same as in Example 1. Compared with the results of Example 1, the extraction rate in the first-stage separation tank decreased, only 1.2 to 1.5%, and no lower fatty acids and phospholipids could be detected in the product, while the content of estradiol and estriol increased; the second-stage separation The extraction rate of the kettle increased to 1.3% to 1.7%, and the content of active ingredients such as linoleic acid, linolenic acid and eicosatetraenoic acid in the product did not change much.

Embodiment 3

[0018] Embodiment 3 utilizes 0.5mol / L ammoniacal liquor and 1mol / L sulfuric acid to prepare the alkaline solution of pH11, as fresh deer antler supercritical CO 2 Extractant for raffinate. The extraction rate of IGF-1 is 3000-3300ng IGF-1 / g fresh velvet, and the growth hormone (GH) in the velvet can also be extracted at the same time, and the GH extraction rate is 20-40ng GH / g fresh velvet.

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Abstract

The comprehensive extraction process of active components in fresh pilose antler includes the first supercritical CO2 extraction at the conditions of temperature 25-35 deg.c, pressure 20-60 MPa and CO2 flow rate 10-25 kg / hr to obtain the active liposoluble components, including fatty acid, estrin, steroid, cholesterol, phosphatide, etc.; and the subsequent ultrasonic lixiviation, ultrafiltering concentration and freeze drying to extract from the supercritical CO2extract the insulin-like growth factor-1 and other active polypeptide. The process of the present invention can realize the comprehensive utilization of two types of active components in pilose antler to raise the application value of pilose antler greatly, and the extracted products may be used in preparing medicine, health article, etc.

Description

technical field [0001] The invention relates to a method for comprehensively extracting active components from fresh velvet antler, which belongs to the technology of extracting active components from velvet antler. Background technique [0002] Antler is the unossified young horns of sika deer or red deer. It is a traditional and precious Chinese medicine in my country. Its therapeutic effect has been recorded in ancient herbal medicines. Modern medicinal chemical analysis shows that deer antler contains a variety of physiologically active ingredients, mainly unsaturated fatty acids, estrogen, steroids, cholesterol, phospholipids, amino acids, polypeptides, polyamines, polysaccharides and hypoxanthine, p-hydroxybenzaldehyde and These active ingredients have various functions such as anti-aging, anti-oxidation, and improving the body's immunity. Proteins and polypeptides in velvet tissue account for more than 50% of the total weight. In recent years, a variety of growth fac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/32
CPCY02P20/54
Inventor 李淑芬王金宇
Owner TIANJIN UNIV
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