Method for measuring plasma calcium by triplex pyridine ruthenium electrochemical luminescence
A ruthenium terpyridine electrochemical, plasma calcium technology, applied in chemiluminescence/bioluminescence, chemical analysis by precipitation, analysis by chemical reaction of materials, etc. Inexpensive, low sample, fast analysis results
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Embodiment 1
[0012] Example 1: Get 100 microliters of plasma and 100 microliters of oxalic acid solution with a concentration of 5 mmol / L and mix evenly so that oxalic acid and blood calcium can fully react, then centrifuge through a centrifuge to make calcium oxalate and macromolecules in blood sink to At the bottom, take the supernatant containing oxalic acid, add ruthenium terpyridyl solution prepared with an acetate buffer solution with a pH value of 5 and mix evenly, then add the mixed solution into the flow injection system for electrochemiluminescence measurement. The calcium content was measured to be 2.478 (±0.053) mmol / L.
Embodiment 2
[0013] Embodiment 2: Carry out comparative test, get 50 microliters of blood plasma and dilute to 100 microliters with the acetate buffer solution of pH value 5.5, mix with 100 microliters concentration and be 5 mmol / liter oxalic acid solution to make oxalic acid and blood calcium fully react, Then centrifuge through a centrifuge to make calcium oxalate and macromolecules in blood sink to the bottom, take the supernatant containing oxalic acid, add ruthenium tertiary pyridine solution prepared with an acetate buffer solution with a pH value of 5.5 and mix evenly, and then mix the mixture Put it into a luminescence cell with a volume of 0.5 ml for static electrochemiluminescence measurement. The calcium content was measured to be 2.496 (±0.054) mmol / L. The calcium content of the same plasma sample measured by atomic absorption spectrometry was 2.531 (±0.029) mmol / L. The plasma calcium content determined by the two methods is quite consistent, and the difference is small.
Embodiment 3
[0014] Embodiment 3: Carry out recovery test, in the blood plasma of 2.48 mmol / liter known calcium amount in 100 microliters, add the calcium of 1.1, 1.5 and 1.9 mmol / liter respectively, make the theoretical value of plasma calcium become 3.58, 3.58, 3.98 and 4.39 mmol / L, and then mixed with the oxalic acid solution with a concentration of 5 mmol / L to make the oxalic acid and calcium react fully, and then centrifuged through the centrifuge to make the calcium oxalate and the macromolecules in the blood sink to the bottom, and take the solution containing To the supernatant of oxalic acid, add ruthenium terpyridyl solution prepared with an acetate buffer solution with a pH value of 7.5 and mix evenly, then put the mixed solution into luminescent cells with a volume of 0.5 ml for static electrochemiluminescence determination. The measured recovery rate is 97.4-102%.
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