Phospholipid modified acrylon nano fiber composite film and its preparation and immobilized enzyme thereof

A technology of nanofibers and immobilized enzymes, applied in the direction of fixing on/in organic carriers, wet spinning, etc., can solve the problems of reduced enzyme activity, low enzyme loading and catalytic efficiency, and difficult recovery of immobilized enzyme carrier materials and other issues to achieve the effect of sufficient sources, improved biological activity and stability, and easy recovery

Inactive Publication Date: 2006-09-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In view of the non-biospecific interaction between some carriers used for immobilizing enzymes and enzymes, which easily leads to the reduction of enzyme activity, as well as the low enzyme loading and catalytic efficiency of immobilized enzymes, and the difficulty of recycling immobilized enzyme carrier materials, etc., The invention provides a phospholipid-modified acrylic nanofiber composite membrane material with high specific surface area, high porosity and good biocompatibility, its preparation and its method for enzyme immobilization

Method used

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  • Phospholipid modified acrylon nano fiber composite film and its preparation and immobilized enzyme thereof

Examples

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example 1

[0034] 4wt% of acrylonitrile / 2-methacryloyloxy-ethyl-phosphatidylcholine copolymer (viscosity-average molecular weight is 80,000, 2-methacryloyloxy-ethyl-phosphatidylcholine content is 1.8 mol%) was dissolved in dimethylformamide, mechanically stirred at 60°C, and completely dissolved to form a homogeneous, transparent, viscous solution; defoaming and removal of impurities to obtain spinning solution, which was added to the injection Connect the spinneret to the spinneret, connect the spinneret to a high-voltage power supply, the distance between the spinneret and the acceptor is about 15 cm, the flow rate of the spinneret spinning solution is 0.2mL / h, and the voltage is 10 k and carry out electrospinning to obtain a phospholipid-modified acrylic nanofiber composite membrane carrier material with a diameter of 60-90 nanometers.

example 2

[0036] 6 wt% of acrylonitrile / 2-methacryloyloxy-ethyl-phosphatidylcholine copolymer (viscosity average molecular weight is 80,000, 2-methacryloyloxy-ethyl-phosphatidylcholine content is 1.8 mol%) was dissolved in dimethylformamide, mechanically stirred at 60°C, and completely dissolved to form a homogeneous, transparent, viscous solution; defoaming and removal of impurities to obtain spinning solution, which was added to the injection Connect the spinneret to the spinneret, connect the spinneret to a high-voltage power supply, the distance between the spinneret and the acceptor is about 15 cm, the flow rate of the spinneret spinning solution is 0.5mL / h, and the voltage is 10 k Volts, electrospinning is carried out to obtain a phospholipid-modified acrylic nanofiber composite membrane carrier material with a diameter of 100-150 nanometers.

example 3

[0038] 4wt% acrylonitrile / 2-methacryloyloxy-ethyl-phosphatidylcholine copolymer (viscosity average molecular weight is 76,000, 2-methacryloyloxy-ethyl-phosphatidylcholine content is 4.9 mol%) was dissolved in dimethylformamide, mechanically stirred at 60°C, and completely dissolved to form a homogeneous, transparent, viscous solution; defoaming and removal of impurities to obtain spinning solution, which was added to the injection Connect the spinneret to the spinneret, connect the spinneret to a high-voltage power supply, the distance between the spinneret and the acceptor is about 15 cm, the flow rate of the spinneret spinning solution is 0.2mL / h, and the voltage is 10 k and carry out electrospinning to obtain a phospholipid modified acrylic nanofiber composite membrane carrier material with a diameter of 50-80 nanometers.

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Abstract

The invention discloses a phosphatide modified acrylic nanometer fibre composite film and preparing and immobilized enzyme method, which comprises the following steps: taking dimethyl sulfoxide, dimethyl formamide or dimethylacetamide as solvent; preparing acrylon copolymer with phosphatide group through electrostatic spinning method as the carrier of immobilized enzyme; immobilizing the enzyme on the nanometer fiber composite film through physical adsorptive method. The invention applies bionic property of high specific surface area, high gap rate and phosphatide property, which produces high enzyme carrying, specific activity and catalyzing rate immobilizing enzyme phosphatide modified acrylic nanometer fibre composite film.

Description

technical field [0001] The invention relates to a phospholipid-modified acrylic nanofiber composite membrane and the technology for preparing and immobilizing enzymes. Background technique [0002] Enzyme is a special kind of protein produced by living cells with high-efficiency catalytic function, high specificity and high controllability. It can specifically promote a certain chemical reaction without participating in the reaction itself. It has high reaction efficiency and mild conditions. The reaction product is less polluted, the energy consumption is low, and the reaction is easy to control, etc., so it is widely used. However, it is prone to denaturation of enzyme protein under the influence of acid, alkali, heat and organic solution, so that the enzyme activity is reduced or lost, and the enzyme reaction is mostly carried out in the solution, it is not easy to recover after the reaction, and the separation and purification of the reaction product is difficult. Moreo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08L33/20C08K5/49C12N11/08D01D5/06
Inventor 徐志康黄小军万灵书
Owner ZHEJIANG UNIV
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