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Bilirubin adsorption material for treating hyperbilirubinemia

A technology for adsorbing material and bilirubin, which is applied in blood circulation treatment, other chemical processes, chemical instruments and methods, etc., can solve the problems of low bilirubin adsorption capacity, high price and low cost, and achieves enhanced safety, Easy to sterilize, small effect of non-specific adsorption

Inactive Publication Date: 2006-12-13
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to design and synthesize a kind of nontoxic, harmless, good bilirubin adsorption effect and low-cost polysaccharide adsorption material, so as to overcome the effect of the adsorption material mainly existing in the prior art on bilirubin in the actual patient's plasma. Defects such as low adsorption capacity and high price

Method used

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  • Bilirubin adsorption material for treating hyperbilirubinemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: N, the preparation of N'carbonyldiimidazole activated agarose gel

[0028] Weigh 0.43~1.0L of agarose gel, vacuum filter 4-5 times with deionized water to remove preservatives in the gel, wash the gel with 30%, 70%, and 100% acetone in turn, weigh 60 Dissolve ~220g of N,N'carbonyldiimidazole in 1.0L of acetone, then add the washed gel into the solution, and react in a shaker at 25~37°C and 150 rpm for 1~2 hours . After the reaction, wash the activated gel repeatedly with 6-10 times the volume of acetone, and vacuum filter to remove the imidazole produced during the reaction. At this time, the active group density on the surface of the agarose gel is 72-192mmol / Lgel.

Embodiment 2

[0029] Example 2: Preparation of bisamino reagent coupled agarose gel

[0030] Add 0.021~0.25L of diamino reagents (such as diaminodipropylimine, hexamethylenediamine, ethylenediamine, etc.) to 1.0L of acetone, then add 0.43~1.0L to activate with N,N'carbonyldiimidazole For a good agarose gel, react in a shaker at a temperature of 25-37°C and 150 rpm for 2-4 hours. After the reaction, wash the reacted gel repeatedly with 6-10 times the volume of deionized water, and then wash the gel sample with 0.15mol / L, pH8.2 boric acid buffer. At this time, the density of amino groups on the surface of the agarose gel is 50-107mmol / Lgel.

Embodiment 3

[0031] Embodiment 3: Preparation of agarose gel with aldehyde active groups

[0032] Disperse 0.43~1.0L of agarose gel coupled with diamino reagents in 1.0L of 0.15mol / L, pH8. 2 in boric acid buffer solution, reacted in a shaker at a temperature of 25-37°C and 150 rpm for 2-18 hours. After the reaction, wash the colloid with 6-10 times the volume of deionized water, 6-10 times the volume of 2mol / L acetic acid, and 6-10 times the volume of 0.15mol / L, pH8. Dry. At this time, the density of aldehyde groups on the surface of the agarose gel is 13-23 mmol / Lgel.

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Abstract

The related bilirubin adsorption material selects agarose gel as carrier activated by N, N'-carbonyldiimidazole, the dual-amido agent and dual-aldehydo agent as separation arm, and couples all of lycine, aethanolamina, arginine and n-butylamine on back end, wherein in 324mg / L blood, the adsorption capacity for former material with different coupled is: 0.71~1.21g / Lgel, 0.78~1.29g / Lgel, 0.91~1.44g / Lgel and 1.20~1.81g / Lgel, and the removal rate to bilirubin by material coupled n-butylamine is up to 50.0%. this invention has well adsorption rate with low cost.

Description

technical field [0001] The invention belongs to the technical field of biological separation engineering. It involves synthesizing a series of bilirubin adsorption materials with lysine, aminoethanol, arginine and n-butylamine adsorption functional groups, and carried out adsorption experiments in patient plasma. Background technique [0002] Bilirubin is the metabolite of heme in aging red blood cells. Under normal circumstances, it is excreted through further metabolism after being combined with glucuronic acid in the liver. If the liver has an obstacle to its metabolism, the bilirubin concentration in the blood will increase, and jaundice will occur when the bilirubin concentration exceeds 17 μmol / L. [0003] At this time, bilirubin has become an endogenous toxin, which can easily pass through biofilms and combine with nerve nuclei through the blood-brain barrier, interfere with the normal metabolism and function of brain cells, and cause irreversible side effects on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22A61M1/38
Inventor 贾凌云
Owner DALIAN UNIV OF TECH
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