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Preparation method and use of separating and purifying lotus leaf flavone and lotus leaf alkaloid by employing polyamide

A technology of lotus leaf alkaloids and lotus leaf flavonoids is applied in chemical instruments and methods, alkaloids, alkaloids and other directions to achieve the effects of reasonable technology, environmental friendliness and simple process.

Inactive Publication Date: 2007-03-07
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The present invention utilizes the specific adsorption characteristics of polyamides to flavonoids to further separate and purify lotus leaf flavonoids and lotus leaf alkaloids to prepare high-purity lotus leaf flavonoids and lotus leaf alkaloids respectively, which has obvious innovation and can provide A preparation method of lotus leaf flavonoids and lotus leaf alkaloids with simple equipment, good separation and purification effect, high product yield and low production cost, which has not been reported in the literature at home and abroad

Method used

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Examples

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Effect test

preparation example Construction

[0028] The preparation method of the lotus leaf crude extract of polar organic solvent extraction is: take lotus leaf as raw material, adopt 30~65% polar organic solvent aqueous solution of 10~20 times of lotus leaf dry weight (W / W), in 50 Extract 1-3 times at ~80°C, 1-3 hours each time, combine the extracts, filter, and recover the solvent under reduced pressure at -0.05~-0.09MPa, 50-65°C, and concentrate in vacuo to form a charge. Leaf crude extract.

[0029] The above-mentioned polar organic solvent can be one or more mixed solvents in methanol, ethanol, propanol, isopropanol, n-butanol or acetone, preferably methanol or acetone. This is mainly based on the fact that the extraction effects of polar organic solvents such as methanol, ethanol, propanol, isopropanol, n-butanol or acetone are equivalent, but compared with other polar organic solvents, methanol or acetone has a lower boiling point and lower energy consumption. Smaller, can significantly reduce the production co...

Embodiment 1

[0056] Using lotus leaves produced in Jianning, Fujian Province as raw materials, wash fresh lotus leaves with water, dry (air-dried, dried or sun-dried), pulverize, and sieve through 16-20 meshes to obtain lotus leaf powder. Put 3.0kg of lotus leaf powder in Put it into the extraction tank, use 40% methanol aqueous solution of 10 times the dry weight (W / W) of lotus leaf powder, extract 3 times under the condition of 63 ℃, each time for 2 hours, combine the extracts, filter, and the filtrate is at -0.05~- Under the conditions of 0.09MPa and 50-65°C, the solvent is recovered under reduced pressure, and the crude extract of lotus leaves is obtained by vacuum concentration. After determination, the extraction rate of lotus leaf flavonoids was 96.7%, and the extraction rate of lotus leaf alkaloids was 95.9%.

[0057] Next, the lotus leaf crude extract is first stirred with an appropriate amount of water to form a suspension, so that the specific gravity of the suspension reaches 1.0...

Embodiment 2

[0062] Put 2.5kg of 16-20 mesh lotus leaf powder into an extraction tank, extract 2 times at 50°C with 30% aqueous methanol solution of 20 times the dry weight (W / W) of lotus leaf powder, each time for 3 hours, and combine The extract is filtered, and the filtrate is recovered under reduced pressure at a temperature of -0.05 to -0.09MPa and 50 to 65°C, and concentrated in a vacuum to form a crude lotus leaf extract. After determination, the extraction rate of lotus leaf flavonoids was 93.4%, and the extraction rate of lotus leaf alkaloids was 92.3%.

[0063] Stir the lotus leaf crude extract with an appropriate amount of water to form a suspension, so that the specific gravity of the suspension reaches 1.18, and then use a polyvinylidene fluoride membrane with a molecular weight cut-off of 60,000 to perform membrane separation and removal of impurities at 0.6 MPa to remove proteins, polysaccharides and other The lotus leaf extract after membrane separation and purification is ...

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Abstract

The invention discloses a preparing method and application of lotus leaf flavone and lotus leaf alkaloid, which comprises the following steps: adopting rough-extract of lotus leaf, film separating impurity-removing lotus leaf extract or resin separating purifying lotus leaf extract as raw material; adding water to stir into suspension; adsorbing lotus leaf flavone through polyamide; decompressing non-adorbed part to recycle; condensing solvent into lotus leaf alkaloid in the vacuum; washing lotus leaf flavone on the polyamide; removing polar impurity and pigment; eluting through polar organic solvent; decompressing eluent; recycling solvent; condensing into lotus leaf flavone in the vacuum; fitting for prepare fat-reducing, antihyperglycemic, antiviral or oxidation resistance oral agent.

Description

technical field [0001] The invention relates to a green chemical preparation method for separating and purifying medicinal active ingredients from plants, and more particularly relates to a preparation method and application of polyamide for separating and purifying lotus leaf flavonoids and lotus leaf alkaloids. Background technique [0002] In China, lotus leaves are rich in resources, cheap and easy to get, and have clear medicinal effects. They are rare bulk natural medicinal and edible plant resources that can be intensively processed and comprehensively developed and utilized. However, in the place of production, a large number of lotus leaves are treated as waste and have not been rationally utilized, resulting in a great waste of resources and a certain degree of environmental pollution. [0003] Nymphaeaceae plant lotus (Nelumbo nucifera Gaertn), a medicinal and edible plant, has been planted in the north and south of my country with a planting history of more than ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/04C07H17/04C07G5/00A61K36/62A61K127/00
Inventor 陈剑锋陈浩郭养浩
Owner FUZHOU UNIV
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